The resistance associated protein RIN4 promotes the extracellular transport of AtEXO70E2

Biochem Biophys Res Commun. 2021 May 28:555:40-45. doi: 10.1016/j.bbrc.2021.03.072. Epub 2021 Mar 31.

Abstract

RIN4 is an important immunomodulator in Arabidopsis, which is targeted by multiple pathogenic effectors, and consequently guarded by different immune receptors. Although RIN4 plays a significant role in plant immunity, its molecular function is not fully understood. We found that RIN4 interacts with the exocyst subunit EXO70E2. Transiently expressed RIN4 can recruits EXO70E2 vesicles to the plasma membrane, and promote the transport of the vesicles to the extracellular matrix. RIN4 also can decrease the protein level of EXO70E2. Base on the fact that EXO70 proteins positively mediates plant immunity, the function of RIN4 is to promote the extracellular export of defense related vesicles. Pathogens will secret effectors to modify or cleavage it to interfere this exocytosis.

Keywords: EXO70E2; Exocytosis; Plant immunity; RIN4.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / cytology
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Cell Membrane / metabolism
  • Intracellular Signaling Peptides and Proteins / genetics
  • Intracellular Signaling Peptides and Proteins / metabolism*
  • Nicotiana / genetics
  • Plant Immunity
  • Plant Leaves / genetics
  • Plant Leaves / metabolism
  • Plants, Genetically Modified
  • Protein Transport
  • Vesicular Transport Proteins / genetics
  • Vesicular Transport Proteins / metabolism*

Substances

  • Arabidopsis Proteins
  • Exo70E2 protein, Arabidopsis
  • Intracellular Signaling Peptides and Proteins
  • RIN4 protein, Arabidopsis
  • Vesicular Transport Proteins