Translocation of HSP47 and generation of mitochondrial reactive oxygen species in human neuroblastoma SK-N-SH cells following electron and X-ray irradiation

Hiroko P Indo; Hiromu Ito; Keiichi Nakagawa; Luksana Chaiswing; Hideyuki J Majima

doi:10.1016/j.abb.2021.108853

Translocation of HSP47 and generation of mitochondrial reactive oxygen species in human neuroblastoma SK-N-SH cells following electron and X-ray irradiation

Arch Biochem Biophys. 2021 May 30:703:108853. doi: 10.1016/j.abb.2021.108853. Epub 2021 Mar 31.

Authors

Hiroko P Indo¹, Hiromu Ito², Keiichi Nakagawa³, Luksana Chaiswing⁴, Hideyuki J Majima⁵

Affiliations

¹ Department of Oncology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, 890-8544, Japan; Amanogawa Galaxy Astronomy Research Center, Kagoshima University Graduate School of Science and Engineering, Kagoshima, 890-0065, Japan. Electronic address: hindoh@dent.kagoshima-u.ac.jp.
² Department of Oncology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, 890-8544, Japan; Amanogawa Galaxy Astronomy Research Center, Kagoshima University Graduate School of Science and Engineering, Kagoshima, 890-0065, Japan.
³ Department of Radiation Oncology, The University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo, Tokyo, 113-8655, Japan.
⁴ Department of Toxicology and Cancer Biology, 454 Bosomworth HSRB, University of Kentucky College of Medicine, Lexington, KY, 40536, USA.
⁵ Department of Oncology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, 890-8544, Japan; Amanogawa Galaxy Astronomy Research Center, Kagoshima University Graduate School of Science and Engineering, Kagoshima, 890-0065, Japan; Department of Toxicology and Cancer Biology, 454 Bosomworth HSRB, University of Kentucky College of Medicine, Lexington, KY, 40536, USA; Department of Space Environmental Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8544, Japan. Electronic address: k0941761@kadai.jp.

PMID: 33811847
DOI: 10.1016/j.abb.2021.108853

Abstract

Generation of mitochondrial reactive oxygen species (ROS), lipid peroxidation, 4-hydroxy-2-nonenal, and heat-shock protein (HSP) 47 after electron and X-ray irradiations were detected in the human neuroblastoma cell line SK-N-SH. After 10 Gy electron irradiation and 15 Gy X-ray irradiation, mitochondrial ROS production and lipid peroxidation were significantly increased. Additionally, we observed a significant increase in the levels of HSP47 after 3 and 10 Gy electron irradiation as well as 15 Gy X-ray irradiation. Furthermore, myristoylation and farnesylation were increased after 10 Gy electron and 15 Gy X-ray irradiations. We found that the level of HSP47 increased in the mitochondria after 10 Gy electron and 15 Gy X-ray irradiations. HSP47 coexisted with myristoylation and farnesylation. Furthermore, HSP47 overexpression increased mitochondrial ROS production. These results suggest that HSP47 plays an important role in mitochondria and induces mitochondrial ROS production in SK-N-SH cells.

Keywords: Electron and X-ray irradiation; Farnesylation; HSP47; Mitochondria; Myristoylation; Reactive oxygen species.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Electrons*
HSP47 Heat-Shock Proteins / metabolism*
Humans
Mitochondria / metabolism*
Mitochondria / radiation effects*
Neuroblastoma / pathology*
Protein Processing, Post-Translational / radiation effects
Protein Transport / radiation effects
Reactive Oxygen Species / metabolism*
X-Rays

Substances

HSP47 Heat-Shock Proteins
Reactive Oxygen Species