Prevention of Covid-19 Infection and Related Complications by Ozonized Oils

Alberto Izzotti; Enzo Fracchia; William Au; Monica Colombo; Ulrich Pfeffer; Laura Emionite; Simone Pavan; Daniele Miotto; Paola Lova; Elena Grasselli; Emanuela Faelli; Ruggeri Piero; Micaela Tiso; Alessandra Pulliero

doi:10.3390/jpm11030226

Prevention of Covid-19 Infection and Related Complications by Ozonized Oils

J Pers Med. 2021 Mar 22;11(3):226. doi: 10.3390/jpm11030226.

Authors

Affiliations

¹ Department of Experimental Medicine, University of Genoa, 16132 Genoa, Italy.
² IRCCS Ospedale Policlinico San Martino, 16132 Genoa, Italy.
³ Galliera Hospital, 16132 Genoa, Italy.
⁴ Faculty of Medicine, Pharmacy, Science and Technology, The George Emil Palade University, 540142 Targu Mures, Romania.
⁵ BWH Graphic Solutions, 28001 Madrid, Spain.
⁶ Department of Chemistry and Industrial Chemistry, University of Genoa, 16132 Genoa, Italy.
⁷ Department of Earth Sciences, University of Genoa, 16132 Genoa, Italy.
⁸ MICAMO Spin-Off Department of Earth Sciences, University of Genoa, 16132 Genoa, Italy.
⁹ Department of Health Sciences, University of Genoa, 16132 Genoa, Italy.

Abstract

Background: The COVID-19 pandemic continues to ravage the human population; therefore, multiple prevention and intervention protocols are being rapidly developed. The aim of our study was to develop a new chemo-prophylactic/-therapeutic strategy that effectively prevents COVID-19 and related complications.

Methods: In in vitro studies, COVID-19 infection-sensitive cells were incubated with human oropharyngeal fluids containing high SARS-CoV-2 loads. Levels of infection were determined via intra-cellular virus loads using quantitative PCR (qPCR). Efficacies for infection prevention were determined using several antiviral treatments: lipid-encapsulated ozonized oil (HOO), water-soluble HOO (HOOws), UV, and hydrogen peroxide. In in vivo studies, safety and efficacy of HOO in fighting COVID-19 infection was evaluated in human subjects.

Results: HOO in combination with HOOws was the only treatment able to fully neutralize SARS-CoV-2 as well as its capacity to penetrate and reproduce inside sensitive cells. Accordingly, the feasibility of using HOO/HOOws was tested in vivo. Analysis of expired gas in healthy subjects indicates that HOO administration increases oxygen availability in the lung. For our human studies, HOO/HOOws was administered to 52 cancer patients and 21 healthy subjects at high risk for COVID-19 infection, and all of them showed clinical safety. None of them developed COVID-19 infection, although an incidence of at least 11 cases was expected. Efficacy of HOO/HOOws was tested in four COVID-19 patients obtaining recovery and qPCR negativization in less than 10 days.

Conclusions: Based on our experience, the HOO/HOOws treatment can be administered at standard doses (three pills per day) for chemo-prophylactic purposes to healthy subjects for COVID-19 prevention and at high doses (up to eight pills per day) for therapeutic purposes to infected patients. This combined prevention strategy can provide a novel protocol to fight the COVID-19 pandemic.

Keywords: COVID-19; COVID-19 challenge test; SARS-CoV-2; chemoprophylaxis; oxidative stress; prevention.