Cell-Mediated Immunoreactivity of Poly(2-isopropenyl-2-oxazoline) as Promising Formulation for Immunomodulation

Ema Paulovičová; Zuzana Kroneková; Lucia Paulovičová; Monika Majerčíková; Juraj Kronek

doi:10.3390/ma14061371

Cell-Mediated Immunoreactivity of Poly(2-isopropenyl-2-oxazoline) as Promising Formulation for Immunomodulation

Materials (Basel). 2021 Mar 12;14(6):1371. doi: 10.3390/ma14061371.

Authors

Ema Paulovičová¹, Zuzana Kroneková², Lucia Paulovičová¹, Monika Majerčíková², Juraj Kronek²

Affiliations

¹ Immunol & Cell Culture Laboratories, Department Immunochemistry of Glycoconjugates, Center of Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia.
² Department for Biomaterials Research, Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 845 41 Bratislava, Slovakia.

Abstract

Poly(2-isopropenyl-2-oxazoline) (PIPOx) represents a functional polymer with high potential for drug delivery, tissue engineering, and immunomodulation. The immunomodulatory efficiency of the PIPOx formulation has been studied in vitro following splenic cells and RAW 264.7 macrophages exposition. The cell-specific immunomodulative effect on production of Th1, Th2, Th17, and Treg signature cytokines has been demonstrated. The impact on the functionality of PIPOx-sensitized RAW 264.7 macrophages was assessed by cell phagocytosis. Time- and concentration-dependent cell internalization and intracellular organelles colocalization of fluorescently labeled PIPOx has been examined. The in vitro results demonstrated the PIPOx bioavailability and the capability of triggering immune cell responses resulting in the induced production of cell-specific signature interleukins, important prerequisite properties for future potential biomedical applications.

Keywords: RAW 264.7; cell internalization; cytokines; immunomodulation; phagocytosis; poly(2-isopropenyl-2-oxazoline).

Abstract

Grants and funding