Generation of a common marmoset embryonic stem cell line CMES40-OC harboring a POU5F1 (OCT4)-2A-mCerulean3 knock-in reporter allele

Sho Yoshimatsu; Rei Murakami; Tsukika Sato; Tsubasa Saeki; Masafumi Yamamoto; Erika Sasaki; Toshiaki Noce; Hideyuki Okano

doi:10.1016/j.scr.2021.102308

Generation of a common marmoset embryonic stem cell line CMES40-OC harboring a POU5F1 (OCT4)-2A-mCerulean3 knock-in reporter allele

Stem Cell Res. 2021 May:53:102308. doi: 10.1016/j.scr.2021.102308. Epub 2021 Mar 24.

Authors

Sho Yoshimatsu¹, Rei Murakami², Tsukika Sato², Tsubasa Saeki², Masafumi Yamamoto³, Erika Sasaki⁴, Toshiaki Noce⁵, Hideyuki Okano¹

Affiliations

¹ Department of Physiology, School of Medicine, Keio University, Tokyo, Japan; Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan.
² Department of Physiology, School of Medicine, Keio University, Tokyo, Japan.
³ ICLAS Monitoring Center, Central Institute for Experimental Animals, Kanagawa, Japan.
⁴ Department of Marmoset Biology and Medicine, Central Institute for Experimental Animals, Kanagawa, Japan.
⁵ Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan.

PMID: 33799281
DOI: 10.1016/j.scr.2021.102308

Abstract

POU class 5 homeobox 1 (POU5F1, also known as OCT4) is critical for maintenance of pluripotency, germ cell fate, reprogramming into a pluripotent state, and early embryogenesis. We generated an embryonic stem cell (ESC) line of the common marmoset (Callithrix jacchus) harboring a heterozygous knock-in allele of OCT4-P2A-mCerulean-T2A-pac. The ESC line (CMES40-OC) will be valuable for investigation of primed/naïve pluripotency and germ cell fate. Homozygous OCT4 knock-in clones were generated but could not be sustained in an undifferentiated state in long-term culture. The OCT4 knock-in system facilitated simultaneous knock-in of a reporter construct at another locus, DDX4 (VASA).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Animals
Callithrix*
Cell Differentiation
Cell Line
Embryonic Stem Cells
Genes, Homeobox*
Octamer Transcription Factor-3 / genetics

Substances

Octamer Transcription Factor-3