ICP-MS-based ionomics method for discriminating the geographical origin of honey of Apis cerana Fabricius

Abstract

The identification of geographical origin is an important factor in evaluating the authenticity of honey. However, at present, there are few studies concerning the honey of Apis cerana Fabricius (A. cerana, Asiatic honeybee). To identify geographical origin, we used two common methods (multi-physicochemical parameters and phenolic chromatographic fingerprints) but achieved only poor identification. To compensate for this shortcoming, we established an ICP-MS-based ionomics method using 18 elements in 27 A. cerana honey samples from three different areas in Shaanxi Province, China. Multivariate analysis showed that significant differences in contents can be used to discriminate the geographical origin of A. cerana honey. The method was further validated using an independent test set of 11 samples with 90.91% accuracy, demonstrating its potential for the identification and prediction of the geographical origin of honey.

Keywords: Apis cerana Fabricius; Geographical origin; Honey; ICP-MS; Ionomics; Mineral element.