Copolymer-nanocapsules of zinc phenyl-thio-phthalocyanine and amphotericin-B in association with antimicrobial photodynamic therapy (A-PDT) applications against Candida albicans yeasts

Rodrigo P Evangelista; Camila F Amantino; Rosemeire C L R Pietro; Rodrigo Sorrechia; Rodolfo D Piazza; Rodrigo F C Marques; Antonio C Tedesco; Fernando L Primo

doi:10.1016/j.pdpdt.2021.102273

Copolymer-nanocapsules of zinc phenyl-thio-phthalocyanine and amphotericin-B in association with antimicrobial photodynamic therapy (A-PDT) applications against Candida albicans yeasts

Photodiagnosis Photodyn Ther. 2021 Jun:34:102273. doi: 10.1016/j.pdpdt.2021.102273. Epub 2021 Mar 30.

Authors

Rodrigo P Evangelista¹, Camila F Amantino¹, Rosemeire C L R Pietro², Rodrigo Sorrechia², Rodolfo D Piazza³, Rodrigo F C Marques³, Antonio C Tedesco⁴, Fernando L Primo⁵

Affiliations

¹ Department of Engineering of Bioprocess and Biotechnology, School of Pharmaceutical Sciences, São Paulo State University - UNESP, Araraquara, São Paulo, 14800-903, Brazil.
² Department of Drugs and Medicines, School of Pharmaceutical Sciences, São Paulo State University - UNESP, Araraquara, 14800-903, São Paulo, Brazil.
³ Laboratory of Magnetic Materials and Colloids, Department of Physical Chemistry, Institute of Chemistry, São Paulo State University, (UNESP), Araraquara, São Paulo, 14801-970, Brazil.
⁴ Department of Chemistry, Center of Nanotechnology and Tissue Engineering - Photobiology and Photomedicine Research Group, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo - USP, Ribeirão Preto, São Paulo, 14010-100, Brazil.
⁵ Department of Engineering of Bioprocess and Biotechnology, School of Pharmaceutical Sciences, São Paulo State University - UNESP, Araraquara, São Paulo, 14800-903, Brazil. Electronic address: fernando.primo@unesp.br.

PMID: 33798749
DOI: 10.1016/j.pdpdt.2021.102273

Abstract

Antimicrobial Photodynamic Therapy (A-PDT) is a modern and non-invasive therapeutic modality. Nanostructures like the polymeric nanocapsules (NC) has proved to be a system that has enormous potential to improve current antimicrobial therapeutic practice. NC of Zinc phenyl-thio-phthalocyanine and Amphotericin B association (NC/ZnS4Pc + AMB) built with poly(lactide-co-glycolide) (PLGA) 50:50 using the preformed polymer interfacial deposition method were developed at a 0.05 mg mL^{- 1} theoretical concentration to improve antifungal activity with two actives association and assistance from PDTa. It showed an average particle diameter of 253.8 ± 17.3, an average polydispersity index of 0.36 ± 0.01, and a negative Zeta potential average of -31.03 ± 5.54 for 158 days. UV-vis absorption and emission spectroscopy analyses did not show changes in photophysical properties in the steady-state of NC/ZnS4Pc + AMB counterparts free ZnS4Pc. The encapsulation percentage of actives was 89.24 % and 7.40 % for ZnS4Pc and AMB, respectively. Cell viability assay using NIH/3T3 ATCC® CRL-1658 ™ cells line showed no cytotoxicity for the concentrations tested. The photodynamic activity assay using NC/ZnS4Pc + AMB diluted showed fungal toxicity against Candida albicans yeast with energetic fluences of 12 J.cm^-2 and 25 J.cm^-2 by a decrease in cell viability. The MFC assay demonstrated a fungistatic activity for the conditions employed in the PDTa assay. The results show that NC/ZnS4Pc + AMB is a promising nanomaterial for antimicrobial inactivation using PDT.

Keywords: Nanocapsules; Photodynamic therapy; Polymeric nanomaterials; Zinc Phenyl-thio-phthalocyanine.

MeSH terms

Amphotericin B
Antifungal Agents / pharmacology
Candida albicans
Indoles
Isoindoles
Nanocapsules*
Photochemotherapy* / methods
Photosensitizing Agents / pharmacology
Polymers
Zinc

Substances

Antifungal Agents
Indoles
Isoindoles
Nanocapsules
Photosensitizing Agents
Polymers
Amphotericin B
Zinc
phthalocyanine