Objective: To observe the effect of ginger-separated moxibustion on fatigue state and intestinal flora in patients with chronic fatigue syndrome (CFS).
Methods: A total of 62 patients with CFS were randomly divided into an observation group (31 cases, 3 cases dropped off) and a control group (31 cases, 2 cases dropped off). The patients in the control group were treated with normal diet and moderate exercise; on the basis of the control group, the patients in the observation group were treated with ginger-separated moxibustion at Zhongwan (CV 12), Shenque (CV 8) and Guanyuan (CV 4), 30 min each time, once every other day, three times a week. Both groups were intervened for 4 weeks. Before and after treatment, the fatigue scale-14 (FS-14) was used to observe the improvement of fatigue state, and 16S rRNA detection technology was used to detect the distribution of intestinal flora.
Results: Compared before treatment, the FS-14 score was reduced after treatment in the observation group (P<0.01), and the reduction in the observation group was larger than that in the control group (P<0.01). The relative abundance of intestinal flora was similar between the observation group and control group at the phylum and genus level before treatment. After treatment, there was no significant change of intestinal flora in the control group. However, the enterobacteriaceae, corynebacterium, erysipelothrix, actinomycetes were increased in the observation group (P<0.05), and actinomycetes, ruminococcus, lactarius had obvious flora advantages compared with the control group (P<0.05).
Conclusion: The ginger-separated moxibustion could significantly improve the fatigue state in CFS patients, which may be related to the regulation of intestinal flora structure and the repair of intestinal barrier.
目的：观察隔姜灸对慢性疲劳综合征（CFS）患者疲劳状态及肠道菌群的影响。方法：将62例CFS患者随机分为观察组（31例，脱落3例）和对照组（31例，脱落2例）。对照组患者正常饮食，适量运动；观察组在对照组基础上于中脘、神阙、关元行隔姜灸治疗，每次30 min，隔日1次，每周3次，两组均干预4周。分别于治疗前后采用疲劳量表14（FS-14）观察两组患者疲劳状态的改善情况，采用16S rRNA检测技术检测肠道菌群分布。结果：观察组治疗后FS-14评分较治疗前降低（P<0.01），且降低幅度大于对照组（P<0.01）。两组治疗前门、属水平菌群相对丰度基本一致。对照组治疗前后无明显菌群差异，观察组治疗后肠杆菌目的肠杆菌科、棒状杆菌科的棒状杆菌属、丹毒丝菌科、放线菌目较治疗前增加（P<0.05），且放线菌属、瘤胃球菌属乳菇属较对照组具有明显菌群优势(P<0.05)。结论：隔姜灸可显著改善CFS 患者的疲劳状态，可能与其调节肠道菌群结构从而修复肠道屏障有关。.
Keywords: chronic fatigue syndrome (CFS); fatigue scale-14 (FS-14); ginger-separated moxibustion; intestinal flora; randomized controlled trial (RCT).