Indiscriminate ssDNA cleavage activity of CRISPR-Cas12a induces no detectable off-target effects in mouse embryos

Protein Cell. 2021 Sep;12(9):741-745. doi: 10.1007/s13238-021-00824-z. Epub 2021 Apr 1.

Yu Wei^#^{1

2}, Yingsi Zhou^#¹, Yajing Liu^#¹, Wenqin Ying¹, Ruiming Lv¹, Qimeng Zhao^{1

2}, Haibo Zhou^{1

3}, Erwei Zuo⁴, Yidi Sun⁵, Hui Yang^{6

7}, Changyang Zhou⁸

¹ Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China.
² University of Chinese Academy of Sciences, Beijing, 100049, China.
³ Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai, 201210, China.
⁴ Center for Animal Genomics, Agricultural Genome Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518124, China. erweizuo@163.com.
⁵ Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China. sydaileen@gmail.com.
⁶ Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China. huiyang@ion.ac.cn.
⁷ Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai, 201210, China. huiyang@ion.ac.cn.
⁸ Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China. zhouchangyang@ion.ac.cn.

^# Contributed equally.

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