Transcardiac Perfusion of the Mouse for Brain Tissue Dissection and Fixation

Jinyun Wu; Yuqi Cai; Xiaoyang Wu; Yue Ying; Yilin Tai; Miao He

doi:10.21769/BioProtoc.3988

Transcardiac Perfusion of the Mouse for Brain Tissue Dissection and Fixation

Bio Protoc. 2021 Mar 5;11(5):e3988. doi: 10.21769/BioProtoc.3988.

Authors

Jinyun Wu¹, Yuqi Cai¹, Xiaoyang Wu¹, Yue Ying¹, Yilin Tai¹, Miao He¹

Affiliation

¹ Institutes of Brain Sciences, Fudan University, Shanghai 200032, China.

Abstract

Transcardiac perfusion with saline followed by 4% paraformaldehyde (PFA) is widely used to clear blood and preserve brain for immunostaining or in situ hybridization. PFA breaks into formaldehyde in solution, which cross-link protein and DNA molecules to preserve tissue and cell structure. Here we provide a step by step guide for performing this procedure in mouse.

Keywords: Aorta; Brain; Mouse; Paraformaldehyde; Transcardiac perfusion.