Facile multifunctional-mode of fabricated biocompatible human serum albumin/reduced graphene oxide/ Cladophora glomerata nanoparticles for bacteriostatic phototherapy, bacterial tracking and antioxidant potential

Nanotechnology. 2021 May 10;32(31). doi: 10.1088/1361-6528/abf457.

Abstract

To overcome multi-drug resistance in microbes, highly efficient antimicrobial substances are required that have a controllable antibacterial effect and are biocompatible. In the present study, an efficient phototherapeutic antibacterial agent, human serum albumin (HSA)/reduced graphene oxide (rGO)/Cladophora glomeratabionanocomposite was synthesized by the incorporation of rGO nanoparticles with HSA, forming protein-rGO, and decorated with a natural freshwater seaweedCladophora glomerata. The prepared HSA/rGO/Cladophora glomeratabionanocomposite was characterized by spectroscopic (UV-vis, FTIR, XRD and Raman) and microscopic (TEM and SEM) techniques. The as-synthesized bionanocomposite showed that sunlight/NIR irradiation stimulated ROS-generating dual-phototherapic effects against antibiotic-resistant bacteria. The bionanocomposite exerted strong antibacterial effects (above 96 %) against amoxicillin-resistantP. aeruginosaandS. aureus, in contrast to single-model-phototherapy. The bionanocomposite not only generated abundant ROS for killing bacteria, but also expressed a fluorescence image for bacterial tracking under sunlight/NIR irradiation. Additionally, the bionanocomposite displayed pronounced antioxidant activity.

Keywords: antioxidant activity; bacterial tracking; bacteriostatic phototherapy; bionanocomposite; photodynamic; photothermal.

MeSH terms

  • Bacterial Load / drug effects
  • Chlorophyta / physiology*
  • Drug Resistance, Multiple, Bacterial / drug effects
  • Graphite / chemistry*
  • HeLa Cells
  • Humans
  • Microbial Viability / drug effects
  • Nanocomposites
  • Oxidative Stress / drug effects*
  • Particle Size
  • Photochemotherapy
  • Photothermal Therapy
  • Pseudomonas aeruginosa / drug effects
  • Pseudomonas aeruginosa / growth & development*
  • Reactive Oxygen Species / metabolism
  • Serum Albumin, Human / chemistry*
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / growth & development*

Substances

  • Reactive Oxygen Species
  • graphene oxide
  • Graphite
  • Serum Albumin, Human