Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells

Weifeng Li; Zhenlin Fan; Yan Lin; Tian-Yun Wang

doi:10.3389/fbioe.2021.646363

Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells

Front Bioeng Biotechnol. 2021 Mar 15:9:646363. doi: 10.3389/fbioe.2021.646363. eCollection 2021.

Authors

Weifeng Li¹, Zhenlin Fan², Yan Lin², Tian-Yun Wang^{1

2}

Affiliations

¹ Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, China.
² International Joint Research Laboratory for Recombinant Pharmaceutical Protein Expression System of Henan, Xinxiang Medical University, Xinxiang, China.

Abstract

At present, nearly 70% of recombinant therapeutic proteins (RTPs) are produced by Chinese hamster ovary (CHO) cells, and serum-free medium (SFM) is necessary for their culture to produce RTPs. In this review, the history and key components of SFM are first summarized, and its preparation and experimental design are described. Some small molecule compound additives can improve the yield and quality of RTP. The function and possible mechanisms of these additives are also reviewed here. Finally, the future perspectives of SFM use with CHO cells for RTP production are discussed.

Keywords: Chinese hamster ovary cells; cell culture; glycosylation; recombinant therapeutic protein; serum free medium.

Publication types

Review