Carbonyl Cyanide 3-Chlorophenylhydrazone (CCCP) Exhibits Direct Antibacterial Activity Against Mycobacterium abscessus

Suting Chen; Tianlu Teng; Zhuman Zhang; Yuanyuan Shang; Hua Xiao; Guanglu Jiang; Fen Wang; Junnan Jia; Lingling Dong; Liping Zhao; Naihui Chu; Hairong Huang

doi:10.2147/IDR.S303113

Carbonyl Cyanide 3-Chlorophenylhydrazone (CCCP) Exhibits Direct Antibacterial Activity Against Mycobacterium abscessus

Infect Drug Resist. 2021 Mar 23:14:1199-1208. doi: 10.2147/IDR.S303113. eCollection 2021.

Authors

Suting Chen^#¹, Tianlu Teng^#^{1

2}, Zhuman Zhang¹, Yuanyuan Shang^{1

2}, Hua Xiao¹, Guanglu Jiang¹, Fen Wang¹, Junnan Jia¹, Lingling Dong¹, Liping Zhao¹, Naihui Chu², Hairong Huang¹

Affiliations

¹ National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing, People's Republic of China.
² Department of Tuberculosis; Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing, People's Republic of China.

^# Contributed equally.

Abstract

Objective: Treatment choices for Mycobacterium abscessus (M. abscessus) infections are very limited, and the prognosis is generally poor. Effective new antibiotics or repurposing existing antibiotics against M. abscessus infection are urgently needed. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP), a member of the lipophilic weak acid class, is known as an efflux pump inhibitor for Mycobacterium tuberculosis. The aim of this study was to determine the inhibitory activity of CCCP as a potential novel antibiotic against M. abscessus.

Methods: A total of 47 reference strains of different mycobacterial species and 60 clinical isolates of M. abscessus were enrolled. In vitro inhibitory activity of CCCP was accessed using microplates alamar blue method with the reference and clinical isolates. The activity of CCCP against intracellular M. abscessus residing within macrophage was also evaluated by intracellular colony numerating assay.

Results: CCCP exhibited good activity against M. abscessus clinical isolates in vitro, the minimum inhibitory concentration (MIC) ranged from 0.47 μg/mL to 3.75 μg/mL, with a MIC₅₀ of 1.875 μg/mL and MIC₉₀ of 3.75 μg/mL. At concentrations safe for the cells, CCCP exhibited highly intracellular bactericidal activities against M. abscessus and M. massiliense reference strains, with inhibitory rates of 84.8%±8.8% and 72.5%±13.7%, respectively. CCCP demonstrated bactericidal activity against intracellular M. abscessus that was comparable to clarithromycin, and concentration-dependent antimicrobial activity against M. abscessus in macrophages was observed. In addition, CCCP also exhibited good activities against most reference strains of rapidly growing mycobacterial species.

Conclusion: CCCP could be a potential candidate of novel antimicrobiological agent to treat M. abscessus infection.

Keywords: Mycobacterium abscessus; carbonyl cyanide 3-chlorophenylhydrazone; intracellular bactericidal activity; minimum inhibitory concentration.

Grants and funding

The study was supported by National Major Science and Technology Projects of China (2018ZX10302-301-004), Beijing Natural Science Foundation (No.5192006), Tongzhou “Yun He” Talent Project (YHLD2018030), and Capital’s Funds for Health Improvement and Research (CFH2020-4-2163), Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support (ZYLX201824), Beijing Hospitals Authority Youth Programme (QML20201601), and Beijing Municipal Administration of Hospitals’ Ascent Plan (DFL20181602).