Recent advances of microelectrode-dot-array (MEDA) based Biochips have revolutionized the application of Lab-on-chip devices. New techniques for MEDA based biochips confide on the concepts on computer-aided design automation and cyberphysical integration to provide ease of use, higher throughput and reliability. One of the major security concerns in MEDA based biochips is actuation tempering attacks targeted to change control sequence daisy chain input resulting in incorrect bioassays. In this paper, we attempted to identify different types of actuation tampering attacks specific to MEDA based biochips. We proposed one technique to detect errors in order to secure the biochips against actuation tempering attacks. This proposed technique is able to monitor such malicious operations and can stall it under any abnormality in operation. Our analysis proves that the proposed method is able to detect errors accurately and proves that this technique is naturally implemented in MEDA.