In-Line Microelectrode Arrays for Impedance Mapping of Microphysiological Systems

Ashlyn T Young; Vladimir A Pozdin; Michael Daniele

doi:10.1109/sensors47125.2020.9278636

In-Line Microelectrode Arrays for Impedance Mapping of Microphysiological Systems

Proc IEEE Sens. 2020 Oct:2020:10.1109/sensors47125.2020.9278636. doi: 10.1109/sensors47125.2020.9278636. Epub 2020 Dec 9.

Authors

Ashlyn T Young¹, Vladimir A Pozdin², Michael Daniele^{1

3}

Affiliations

¹ Joint Department of Biomedical Engineering, NC State University and UNC at Chapel Hill, Raleigh, NC, USA.
² Department of Electrical and Computer Engineering, Florida International University, Miami, FL, USA.
³ Department of Electrical and Computer Engineering, NC State University, Raleigh, NC, USA.

Abstract

Herein, a 60-electrode array is fabricated down the length of a microchamber for analysis of a microphysiological system. The electrode array is fabricated by standard photolithographic, metallization, and etching techniques. Permutations of 2-wire impedance measurements (10 Hz to 1 MHz) are made along the length of the microchannel using a multiplexer, Gamry potentiostat, and custom Labview code. An impedance "heat map" is created via custom algorithms. Spatial resolution and mapping capabilities are exhibited using conductive NaCl solutions and 2D cell culture.

Keywords: cell culture; impedance mapping; microphysiological system; transendothelial electrical resistance.

Grants and funding

T32 HL069768/HL/NHLBI NIH HHS/United States