Treatment of giant congenital melanocytic nevi with cultured epithelial autografts: Clinical and histopathological analysis

A Shoji-Pietraszkiewicz; M Sakamoto; M Katsube; S Ogino; I Tsuge; H Yamanaka; J Arata; N Morimoto

doi:10.1016/j.reth.2021.02.003

Treatment of giant congenital melanocytic nevi with cultured epithelial autografts: Clinical and histopathological analysis

Regen Ther. 2021 Mar 14:18:1-6. doi: 10.1016/j.reth.2021.02.003. eCollection 2021 Dec.

Authors

A Shoji-Pietraszkiewicz¹, M Sakamoto¹, M Katsube¹, S Ogino¹, I Tsuge¹, H Yamanaka¹, J Arata², N Morimoto¹

Affiliations

¹ Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, 54 Shogoin, Kawahara-cho, Sakyou-ku, Kyoto, 606-8507, Japan.
² Department of Plastic and Reconstructive Surgery, National Hospital Organization Kyoto Medical Center, 1-1 Mukaihata-cho, Fukakusa, Fushimiku, Kyoto, 612-8555, Japan.

Abstract

Introduction: Curettage and dermabrasion are effective in the treatment of giant congenital melanocytic nevi (GCMN); however, local infection and hypertrophic scar formation are major issues. Thus, we applied cultured epithelial autografts (CEA) on skin defects after curettage or abrasion of GCMN and assessed the postoperative outcomes.

Methods: Seven nevi lesions of five patients (aged 3 months to 24 years) were treated with CEA after curettage or abrasion with a dermatome or a surgical bar, respectively. We assessed the postoperative outcomes, including CEA take ratio, erosion and/or ulcer formation in the acute phase, hospitalization days, Vancouver scar scale, and color improvement one year after the operation. In addition, a histological evaluation of a skin biopsy was performed over one year after the operation.

Results: The CEAs took well on the wound, and the wound surface was mostly epithelized by postoperative day 7 in all cases. While hypertrophic scar formation and slight pigmentation were observed in some lesions, the color was improved in all of the treated lesions. Histopathological examination revealed that the regenerated epidermis had stratified keratinocytes with rete ridges, and the dermal layer without nevus cells regenerated above the remaining dermis layer.

Conclusions: In this study, we found that early epithelialization and regeneration of the dermal layer was achieved after the application of CEA, suggesting that CEA could be an effective option after curettage or abrasion of GCMN.

Keywords: CEA, cultured epithelial autograft; CMN, congenital melanocytic nevi; Cultured epidermis; Curettage; Dermal regeneration; GCMN, giant congenital melanocytic nevi; Giant congenital melanocytic nevus.