Derrone Inhibits Platelet Aggregation, Granule Secretion, Thromboxane A2 Generation, and Clot Retraction: An In Vitro Study

Jung-Hae Shin; Muhammad Irfan; Man Hee Rhee; Hyuk-Woo Kwon

doi:10.1155/2021/8855980

Derrone Inhibits Platelet Aggregation, Granule Secretion, Thromboxane A₂ Generation, and Clot Retraction: An In Vitro Study

Evid Based Complement Alternat Med. 2021 Mar 10:2021:8855980. doi: 10.1155/2021/8855980. eCollection 2021.

Authors

Jung-Hae Shin¹, Muhammad Irfan^{2

3}, Man Hee Rhee², Hyuk-Woo Kwon⁴

Affiliations

¹ Department of Biomedical Laboratory Science, Catholic Kwandong University, Gangneung 25601, Republic of Korea.
² Laboratory of Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea.
³ Department of Oral Biology, University of Illinois at Chicago, Chicago 60607, IL, USA.
⁴ Department of Biomedical Laboratory Science, Far East University, Eumseong 27601, Republic of Korea.

Abstract

Cudrania tricuspidata (C. tricuspidata) is widespread throughout East Asia and in China and Korea, and it is widely used as a traditional remedy against eczema, mumps, and tuberculosis. With regard to the aforementioned medical efficacy, various studies are continuously being conducted, and it has been reported that C. tricuspidata extract has various actions against inflammation, diabetes, obesity, and tumors. Therefore, we evaluated antiplatelet effects using derrone in C. tricuspidata. We examined the effect of derrone on the phosphorylation of vasodilator-stimulated phosphoprotein (VASP) and inositol 1, 4, 5-triphosphate receptor I (IP₃RI), and on the dephosphorylation of cytosolic phospholipase A₂ (cPLA₂), mitogen-activated protein kinases p38 (p38^MAPK), and Akt, which affects platelet function and thrombus formation. Various agonists-induced human platelets were inhibited by derrone without cytotoxicity, and it also decreased the intracellular calcium level through the signaling molecule phosphorylations. In addition, derrone inhibited glycoprotein IIb/IIIa (αIIb/β3) affinity. Thus, in the present study, derrone suppressed human platelet aggregation and thrombin-induced clot formation.