Progression and application of CRISPR-Cas genomic editors

Li Yang; Jing Tang; Xuelei Ma; Yuan Lin; Guorong Ma; Minghai Shan; Libin Wang; Yanhui Yang

doi:10.1016/j.ymeth.2021.03.013

Progression and application of CRISPR-Cas genomic editors

Methods. 2021 Oct:194:65-74. doi: 10.1016/j.ymeth.2021.03.013. Epub 2021 Mar 25.

Authors

Li Yang¹, Jing Tang¹, Xuelei Ma¹, Yuan Lin¹, Guorong Ma¹, Minghai Shan², Libin Wang³, Yanhui Yang⁴

Affiliations

¹ Basic Medical School, Ningxia Medical University, Yinchuan, People's Republic of China.
² General Hospital of Ningxia Medical University, Yinchuan, People's Republic of China.
³ General Hospital of Ningxia Medical University, Yinchuan, People's Republic of China. Electronic address: wanglibin007@126.com.
⁴ Basic Medical School, Ningxia Medical University, Yinchuan, People's Republic of China. Electronic address: yangyanhui@nxmu.edu.cn.

PMID: 33774156
DOI: 10.1016/j.ymeth.2021.03.013

Abstract

Base editing technology is an efficient tool for genome editing, particularly in the correction of base mutations. Diverse base editing systems were developed according to the dCas9 or nCas9 linked with different deaminase or reverse transcriptase in the editors, including ABEs, CBEs, PEs and dual-functional of base editor (such as CGBE1, A&C-BEmax, ACBE, etc.). Currently, Base editing technology has been widely applied to various fields such as microorganisms, plants, animals and medicine for basic research and therapeutics. Here, we reviewed the advancement of base editing technology. We also discussed the application of base editors in different areas in the future.

Keywords: Base editors; CRISPR/Cas9; Dual-functional base editors; Genome editing; Prime editors.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CRISPR-Cas Systems* / genetics
Gene Editing*
Genomics
Mutation