Raising the Bar(-seq) in Leishmania Genetic Screens

Tiago R Ferreira; Rafael M Couñago; Nilmar S Moretti

doi:10.1016/j.pt.2021.03.002

Raising the Bar(-seq) in Leishmania Genetic Screens

Trends Parasitol. 2021 May;37(5):367-369. doi: 10.1016/j.pt.2021.03.002. Epub 2021 Mar 24.

Authors

Tiago R Ferreira¹, Rafael M Couñago², Nilmar S Moretti³

Affiliations

¹ Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institute of Health, Bethesda, MD, USA. Electronic address: tiago.rodriguesferreira@nih.gov.
² Centro de Química Medicinal (CQMED), Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas (UNICAMP), Campinas, SP 13083-875, Brazil; Structural Genomics Consortium, Departamento de Genética e Evolução, Instituto de Biologia, UNICAMP, Campinas, SP13083-886, Brazil.
³ Laboratório de Biologia Molecular de Patógenos (LBMP), Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil.

PMID: 33773911
DOI: 10.1016/j.pt.2021.03.002

Abstract

Our understanding of regulatory factors in Leishmania differentiation has long been restricted by the available genetic tools, but the availability of CRISPR/Cas9 has changed the landscape forever. Recently, Baker and Catta-Preta et al. applied Cas9 editing and kinome-wide bar-seq to dissect the function of 204 kinases in the Leishmania mexicana life cycle.

Keywords: Cas9; Leishmania; NGS; bar-seq; kinase; kinome.

Published by Elsevier Ltd.

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MeSH terms

Animals
CRISPR-Cas Systems
Leishmania* / genetics
Life Cycle Stages