Altered sphingolipid function in Alzheimer's disease; a gene regulatory network approach

Neurobiol Aging. 2021 Jun:102:178-187. doi: 10.1016/j.neurobiolaging.2021.02.001. Epub 2021 Feb 7.

Abstract

Sphingolipids (SLs) are bioactive lipids involved in various important physiological functions. The SL pathway has been shown to be affected in several brain-related disorders, including Alzheimer's disease (AD). Recent evidence suggests that epigenetic dysregulation plays an important role in the pathogenesis of AD as well. Here, we use an integrative approach to better understand the relationship between epigenetic and transcriptomic processes in regulating SL function in the middle temporal gyrus of AD patients. Transcriptomic analysis of 252 SL-related genes, selected based on GO term annotations, from 46 AD patients and 32 healthy age-matched controls, revealed 103 differentially expressed SL-related genes in AD patients. Additionally, methylomic analysis of the same subjects revealed parallel hydroxymethylation changes in PTGIS, GBA, and ITGB2 in AD. Subsequent gene regulatory network-based analysis identified 3 candidate genes, that is, SELPLG, SPHK1 and CAV1 whose alteration holds the potential to revert the gene expression program from a diseased towards a healthy state. Together, this epigenomic and transcriptomic approach highlights the importance of SL-related genes in AD, and may provide novel biomarkers and therapeutic alternatives to traditionally investigated biological pathways in AD.

Keywords: Alzheimer's disease; Disease network analysis; Epigenetics; Gene regulatory network; Sphingolipids.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing / genetics
  • Adaptor Proteins, Signal Transducing / metabolism
  • Alzheimer Disease / genetics*
  • Caveolin 1 / genetics
  • Caveolin 1 / metabolism
  • Epigenesis, Genetic / genetics*
  • Female
  • Gene Expression
  • Gene Expression Profiling
  • Gene Regulatory Networks / genetics*
  • Genetic Association Studies*
  • Humans
  • Male
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism
  • Methylation
  • Sphingolipids / genetics*
  • Sphingolipids / metabolism
  • Sphingolipids / physiology
  • Temporal Lobe / metabolism
  • Transcriptome / genetics

Substances

  • Adaptor Proteins, Signal Transducing
  • CAV1 protein, human
  • Caveolin 1
  • Membrane Glycoproteins
  • P-selectin ligand protein
  • SPHKAP protein, human
  • Sphingolipids