Differentiating Induced Pluripotent Stem Cells Toward Mesenchymal Stem/Stromal Cells

Shennea S McGarvey; Michael Ferreyros; Igor Kogut; Ganna Bilousova

doi:10.1007/7651_2021_383

Differentiating Induced Pluripotent Stem Cells Toward Mesenchymal Stem/Stromal Cells

Methods Mol Biol. 2022:2549:153-167. doi: 10.1007/7651_2021_383.

Authors

Shennea S McGarvey^{1

2}, Michael Ferreyros^{1

2}, Igor Kogut^{1

2}, Ganna Bilousova^{3

4

5}

Affiliations

¹ Department of Dermatology, University of Colorado School of Medicine, Aurora, CO, USA.
² Charles C. Gates Center for Regenerative Medicine, University of Colorado School of Medicine, Aurora, CO, USA.
³ Department of Dermatology, University of Colorado School of Medicine, Aurora, CO, USA. Ganna.Bilousova@CUAnschutz.edu.
⁴ Charles C. Gates Center for Regenerative Medicine, University of Colorado School of Medicine, Aurora, CO, USA. Ganna.Bilousova@CUAnschutz.edu.
⁵ Linda Crnic Institute for Down Syndrome, University of Colorado School of Medicine, Aurora, CO, USA. Ganna.Bilousova@CUAnschutz.edu.

PMID: 33772462
DOI: 10.1007/7651_2021_383

Abstract

Differentiating human induced pluripotent stem cells (iPSCs) into multipotent mesenchymal stem/stromal cells (MSCs) offers a renewable source of therapeutically invaluable cells. However, the process of MSC derivation from iPSCs suffers from an undesirably low efficiency. In this chapter, we present an optimized procedure to produce MSCs from human iPSCs with a high efficiency. The protocol depends on the generation of embryoid bodies (EBs) and requires the treatment of EBs with transforming growth factor beta 1 (TGF-β1). The resulting MSCs can be purified based on the expression of CD73, CD105, and CD90 markers and expanded for multiple passages without losing their characteristics.

Keywords: Differentiation; Induced pluripotent stem cells; MSCs; Mesenchymal stem/stromal cells; iPSCs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Embryoid Bodies / metabolism
Humans
Induced Pluripotent Stem Cells* / metabolism
Mesenchymal Stem Cells* / metabolism
Thy-1 Antigens / metabolism

Substances

Thy-1 Antigens