Poly(2-allylamidopropyl-2-oxazoline)-Based Hydrogels: From Accelerated Gelation Kinetics to In Vivo Compatibility in a Murine Subdermal Implant Model

Tim R Dargaville; Damien G Harkin; Jong-Ryul Park; Amanda Cavalcanti; Eleonore C L Bolle; Flavia Medeiros Savi; Brooke L Farrugia; Bryn D Monnery; Yann Bernhard; Joachim F R Van Guyse; Annelore Podevyn; Richard Hoogenboom

doi:10.1021/acs.biomac.1c00046

Poly(2-allylamidopropyl-2-oxazoline)-Based Hydrogels: From Accelerated Gelation Kinetics to In Vivo Compatibility in a Murine Subdermal Implant Model

Biomacromolecules. 2021 Apr 12;22(4):1590-1599. doi: 10.1021/acs.biomac.1c00046. Epub 2021 Mar 25.

Authors

Tim R Dargaville^{1

2

3}, Damien G Harkin^{3

4}, Jong-Ryul Park^{1

2

3}, Amanda Cavalcanti^{1

2

3}, Eleonore C L Bolle^{1

2

3}, Flavia Medeiros Savi³, Brooke L Farrugia⁵, Bryn D Monnery⁶, Yann Bernhard⁶, Joachim F R Van Guyse⁶, Annelore Podevyn⁶, Richard Hoogenboom⁶

Affiliations

¹ Centre for Materials Science, Queensland University of Technology, 2 George Street, Brisbane, Queensland 4000, Australia.
² School of Chemistry and Physics, Queensland University of Technology, 2 George Street, Brisbane, Queensland 4000, Australia.
³ Institute of Health and Biomedical Innovation, Science and Engineering Faculty, Queensland University of Technology, Brisbane, Queensland 4001, Australia.
⁴ School of Biomedical Sciences, Queensland University of Technology, 2 George Street, Brisbane, Queensland, 4000, Australia.
⁵ Department of Biomedical Engineering, The University of Melbourne, Melbourne, Victoria 3010, Australia.
⁶ Supramolecular Chemistry Group, Centre of Macromolecular Chemistry (CMaC), Department of Organic and Macromolecular Chemistry, Ghent University, Krijgslaan 281 S4, B-9000 Ghent, Belgium.

PMID: 33764748
DOI: 10.1021/acs.biomac.1c00046

Abstract

A rapid photo-curing system based on poly(2-ethyl-2-oxazoline-co-2-allylamidopropyl-2-oxazoline) and its in vivo compatibility are presented. The base polymer was synthesized from the copolymerization of 2-ethyl-2-oxazoline (EtOx) and the methyl ester containing 2-methoxycarboxypropyl-2-oxazoline (C₃MestOx) followed by amidation with allylamine to yield a highly water-soluble macromer. We showed that spherical hydrogels can be obtained by a simple water-in-oil gelation method using thiol-ene coupling and investigated the in vivo biocompatibility of these hydrogel spheres in a 28-day murine subdermal model. For comparison, hydrogel spheres prepared from poly(ethylene glycol) were also implanted. Both materials displayed mild, yet typical foreign body responses with little signs of fibrosis. This is the first report on the foreign body response of a poly(2-oxazoline) hydrogel, which paves the way for future investigations into how this highly tailorable class of materials can be used for implantable hydrogel devices.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Hydrogels*
Kinetics
Mice
Polyethylene Glycols*
Polymerization
Polymers

Substances

Hydrogels
Polymers
Polyethylene Glycols