Photodynamic effect of TPP encapsulated in polystyrene nanoparticles toward multi-resistant pathogenic bacterial strains: AFM evaluation

Sci Rep. 2021 Mar 24;11(1):6786. doi: 10.1038/s41598-021-85828-9.

Abstract

Photodynamic inactivation (PDI) is a promising approach for the efficient killing of pathogenic microbes. In this study, the photodynamic effect of sulfonated polystyrene nanoparticles with encapsulated hydrophobic 5,10,15,20-tetraphenylporphyrin (TPP-NP) photosensitizers on Gram-positive (including multi-resistant) and Gram-negative bacterial strains was investigated. The cell viability was determined by the colony forming unit method. The results showed no dark cytotoxicity but high phototoxicity within the tested conditions. Gram-positive bacteria were more sensitive to TPP-NPs than Gram-negative bacteria. Atomic force microscopy was used to detect changes in the morphological properties of bacteria before and after the PDI treatment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / drug effects*
  • Bacteria / radiation effects*
  • Drug Compounding*
  • Microscopy, Atomic Force
  • Nanoparticles* / chemistry
  • Photochemical Processes*
  • Photochemotherapy / methods
  • Polystyrenes* / chemistry
  • Porphyrins / administration & dosage*
  • Porphyrins / chemistry*

Substances

  • Polystyrenes
  • Porphyrins
  • tetraphenylporphyrin