Lipidomics phenotyping of clam (Corbicula fluminea) through graphene/fibrous silica nanohybrids based solid-phase extraction and HILIC-MS analysis

Gongshuai Song; Qinchao Zhu; Linqiu Li; Zhenxiao Zheng; Qiaoling Zhao; Junli Feng; Xiaodi Zhang; Pingya Wang; Kang Chen; Qing Shen

doi:10.1016/j.foodchem.2021.129565

Lipidomics phenotyping of clam (Corbicula fluminea) through graphene/fibrous silica nanohybrids based solid-phase extraction and HILIC-MS analysis

Food Chem. 2021 Aug 30:354:129565. doi: 10.1016/j.foodchem.2021.129565. Epub 2021 Mar 13.

Authors

Gongshuai Song¹, Qinchao Zhu², Linqiu Li³, Zhenxiao Zheng¹, Qiaoling Zhao⁴, Junli Feng¹, Xiaodi Zhang¹, Pingya Wang⁴, Kang Chen⁵, Qing Shen⁶

Affiliations

¹ Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China.
² Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou, China.
³ School of Public Health, Guangdong Medical University, Dongguan, China.
⁴ Zhoushan Institute of Calibration and Testing for Quality and Technical Supervision, Zhoushan 316021, China.
⁵ Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China. Electronic address: chenkangfood@163.com.
⁶ Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China. Electronic address: sq@zjgsu.edu.cn.

PMID: 33756323
DOI: 10.1016/j.foodchem.2021.129565

Abstract

Polyunsaturated phospholipids are abundant in clam (Corbicula fluminea) but difficult to be fully extracted. Herein, graphene/fibrous silica (G/KCC-1) nanohybrids were synthesized, characterized, and applied for solid-phase extraction (SPE) of phospholipids in clam. The effectiveness of G/KCC-1 SPE was verified by hydrophilic interaction chromatography mass spectrometry (HILIC-MS) based lipidomics and statistical analysis. The ions of PE 16:0/18:1 (m/z 716.4), PC 16:0/20:5 (m/z 824.6) and etc. were regarded as the main difference among the crude lipids, acetone washed extract, and eluate of G/KCC-1 SPE. Finally, this method was validated in terms of linearity (R² 0.9965 to 0.9981), sensitivity (LOD 0.19-0.51 μg·mL^-1 and LOQ 0.48 - 1.47 μg·mL^-1), and precision (RSD_intra-day ≤ 7.16% and RSD_inter-day ≤ 7.30%). In conclusion, the G/KCC-1 SPE and HILIC-MS method was shown to be accurate and efficient in selective extracting and phenotyping phospholipids in C. fluminea.

Keywords: Corbicula fluminea; Graphene/KCC-1; HILIC-MS; Phospholipids; Solid-phase extraction.

MeSH terms

Animals
Chromatography, Liquid*
Corbicula / metabolism*
Graphite / chemistry*
Hydrophobic and Hydrophilic Interactions
Lipidomics / methods*
Mass Spectrometry*
Phenotype
Seafood / analysis
Silicon Dioxide / chemistry*
Solid Phase Extraction*

Substances

Silicon Dioxide
Graphite