Next Generation of LFD Testing - Smartphone-Based Mycotoxin Analysis with RIDA®SMART APP

J AOAC Int. 2021 Mar 5;104(1):49-52. doi: 10.1093/jaoacint/qsaa117.

Abstract

Background: Grain mills, feed mills, ethanol plants, flour producers, but also mobile laboratory services need simple and reliable possibilities to analyze grain for mycotoxins during harvest in the field.

Objective: The RIDA®SMART APP is a smartphone application, which was developed together with a Lateral Flow Device as a quantitative and fast on-site/on-field tool for mycotoxin analyses.

Method: It is a small and light device with its own power supply, very versatile, flexible and easy to use. For quantification, a lot specific calibation curve is uploaded to the RIDA®SMART APP via a QR code from the certificate of analysis. The software application in combination with a compatible smartphone, analyzes the LFD strip via a picture taken with a smartphone and quantifies the mycotoxin contamination within 4 second. Due to the possibility to use mobile networks or WIFI, there are no difficulties in the organization of the collected results. Currently, nine different android-based smartphones from various manufacturers are compatible with the RIDA®SMART APP.

Results: In combination with one of the compatible smartphones, the RIDA®SMART APP is able to generate results with a coefficient of variation CV ≤ 13%, even at highly varying lighting conditions. The recovery of certified reference corn samples, previously analyzed with HPLC or LC-MS/MS, shows a mean value between 91% - 119%.

Conclusion: The RIDA®SMART APP is highly suitable for an inexpensive, quick and accurate quantitative measurement of mycotoxins in agricultural products (e.g. aflatoxins in corn).

Highlights: To our best knowledge, the RIDA®SMART APP is the only smartphone-based method for quantitative mycotoxin analysis worldwide combined with a broader measuring range as most usual lateral flow analyzers.

MeSH terms

  • Animals
  • Chromatography, Liquid
  • Mobile Applications*
  • Mycotoxins*
  • Scrapie*
  • Sheep
  • Smartphone
  • Tandem Mass Spectrometry

Substances

  • Mycotoxins