Data on the uptake of CpG-loaded amino-dextran nanoparticles by antigen-presenting cells

Hien V Nguyen; Katrin Campbell; Gavin F Painter; Sarah L Young; Greg F Walker

doi:10.1016/j.dib.2021.106883

Data on the uptake of CpG-loaded amino-dextran nanoparticles by antigen-presenting cells

Data Brief. 2021 Feb 25:35:106883. doi: 10.1016/j.dib.2021.106883. eCollection 2021 Apr.

Authors

Hien V Nguyen^{1

2}, Katrin Campbell², Gavin F Painter³, Sarah L Young⁴, Greg F Walker¹

Affiliations

¹ School of Pharmacy, University of Otago, Dunedin 9016, New Zealand.
² Department of Pathology, University of Otago, Dunedin 9016, New Zealand.
³ The Ferrier Research Institute, Victoria University of Wellington, Wellington 5040, New Zealand.
⁴ School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Sydney 2006, Australia.

Abstract

Cytosine-phosphate-guanine (CpG) oligonucleotides are commonly-used vaccine adjuvants to promote the activation of antigen-presenting cells (APCs). To mount an effective immune response, CpG needs to be internalized and bind to its endosomal Toll-like receptor 9 (TLR-9) inside the APCs. Using flow cytometry and fluorescence microscopy, this article presents the cellular uptake data of the amino-dextran nanoparticle (aDNP) and aDNP loaded with CpG immobilized on its surface by either electrostatic adsorption or covalent conjugation. The uptake of fluorescently-labelled aDNPs by murine splenic dendritic cells and macrophages was determined by flow cytometry and uptake by murine bone-marrow-derived dendritic cells was evaluated by fluorescence microscopy. The data presented in this paper correlates with the in vitro immune-stimulatory activity observed for the two different CpG loading methods in the research article "Nanoparticle system based on amino-dextran as a drug delivery vehicle: immune-stimulatory CpG-oligonucleotide loading and delivery" (Nguyen et al., 2020) [1]. The data provide experimental evidence for a better understanding how the nanoparticle surface loading method of CpG influences the uptake of these nanoparticles by antigen-presenting cells as a step guide in the design of more effective vaccine formulations.

Keywords: Cellular uptake; Covalent conjugation; CpG oligonucleotides; Dextran nanoparticle; Electrostatic adsorption; Flow cytometry; Fluorescence microscopy; Vaccine formulation.