In vitro activity of encapsulated lactic acid bacteria on aflatoxin production and growth of Aspergillus Spp

Rouhollah Mohammadi; Sepideh Abbaszadeh; Aghil Sharifzadeh; Mojtaba Sepandi; Maryam Taghdir; Najmeh Youseftabar Miri; Karim Parastouei

doi:10.1002/fsn3.2015

In vitro activity of encapsulated lactic acid bacteria on aflatoxin production and growth of Aspergillus Spp

Food Sci Nutr. 2021 Jan 19;9(3):1282-1288. doi: 10.1002/fsn3.2015. eCollection 2021 Mar.

Authors

Rouhollah Mohammadi¹, Sepideh Abbaszadeh^{1

2}, Aghil Sharifzadeh³, Mojtaba Sepandi¹, Maryam Taghdir¹, Najmeh Youseftabar Miri⁴, Karim Parastouei¹

Affiliations

¹ Health Research Center Life style institute Baqiyatallah University of Medical Sciences Tehran Iran.
² Department of Nutrition and Food Hygiene Faculty of Health Baqiyatallah University of Medical Sciences Tehran Iran.
³ Department of Microbiology and Immunology, Faculty of Veterinary Medicine University of Tehran Tehran Iran.
⁴ Department of Food Science Faculty of Agriculture Ferdowsi University Mashhad Iran.

Abstract

This study aimed to investigate the potential ability of simultaneously used L. acidophilus(LA-5), L.rhamnosus(LGG), and L.casei(LC-01) in encapsulated (E) and nonencapsulated (NE) forms in mycelial growth of Aspergillus spp and aflatoxin production by A. flavus. In order to assess the zone of fungal growth inhibition by E and NE lactic acid bacteria, the agar well diffusion method was applied. Quantification of aflatoxin was performed using a high-performance liquid chromatography technique. Lactic acid bacteria exhibited high antifungal activity and significantly reduced AFB1, AFB2, AFG1, and AFG2 production in both E and NE forms compared to the control group. The percentage of reduction in total AFs production in treated samples with E and NE lactic acid bacteria was 94.1% and 95.5%, respectively. These results suggested that simultaneously used lactic acid bacteria in E and NE forms can prevent growth and decrease aflatoxin production of toxigenic aspergilla.

Keywords: Aflatoxin; Antifungal activity; Aspergillus; Lactic acid bacteria.