Deletion Mutants, Archived Transposon Library, and Tagged Protein Constructs of the Model Sulfate-Reducing Bacterium Desulfovibrio vulgaris Hildenborough

Judy D Wall; Grant M Zane; Thomas R Juba; Jennifer V Kuehl; Jayashree Ray; Swapnil R Chhabra; Valentine V Trotter; Maxim Shatsky; Kara B De León; Kimberly L Keller; Kelly S Bender; Gareth Butland; Adam P Arkin; Adam M Deutschbauer

doi:10.1128/MRA.00072-21

Deletion Mutants, Archived Transposon Library, and Tagged Protein Constructs of the Model Sulfate-Reducing Bacterium Desulfovibrio vulgaris Hildenborough

Microbiol Resour Announc. 2021 Mar 18;10(11):e00072-21. doi: 10.1128/MRA.00072-21.

Affiliations

¹ Biochemistry Division, University of Missouri, Columbia, Missouri, USA wallj@missouri.edu.
² Biochemistry Division, University of Missouri, Columbia, Missouri, USA.
³ Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
⁴ Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
⁵ Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.

Abstract

The dissimilatory sulfate-reducing deltaproteobacterium Desulfovibrio vulgaris Hildenborough (ATCC 29579) was chosen by the research collaboration ENIGMA to explore tools and protocols for bringing this anaerobe to model status. Here, we describe a collection of genetic constructs generated by ENIGMA that are available to the research community.