Measurement of intraorgan fat and hepatic output of triglycerides in human type 2 diabetes by magnetic resonance and intralipid infusion techniques

STAR Protoc. 2021 Mar 2;2(1):100355. doi: 10.1016/j.xpro.2021.100355. eCollection 2021 Mar 19.

Abstract

Liver fat content and the linked rate of export of triglyceride are central to the etiology of type 2 diabetes, as well as to the cardiovascular effects of fatty liver disease. Measurement in humans of intrahepatic and intrapancreatic fat content is described using magnetic resonance techniques and quantification of the rate of hepatic secretion of very low density lipoprotein using a non-isotopic competitive blocking of tissue uptake. This protocol is non-invasive, can be repeated sequentially, and does not involve ionizing radiation. For complete details on the use and execution of this protocol, please refer to (Taylor et al., 2018) and (Al-Mrabeh et al., 2020b).

Keywords: Clinical protocol; Metabolism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Diabetes Mellitus, Type 2* / diagnostic imaging
  • Diabetes Mellitus, Type 2* / metabolism
  • Emulsions / administration & dosage
  • Fatty Liver* / diagnostic imaging
  • Fatty Liver* / metabolism
  • Humans
  • Lipoproteins, VLDL / metabolism*
  • Liver / diagnostic imaging
  • Liver / metabolism
  • Magnetic Resonance Imaging*
  • Male
  • Pancreas* / diagnostic imaging
  • Pancreas* / metabolism
  • Phospholipids / administration & dosage*
  • Soybean Oil / administration & dosage*

Substances

  • Emulsions
  • Lipoproteins, VLDL
  • Phospholipids
  • soybean oil, phospholipid emulsion
  • Soybean Oil