Design and Construction of Repacked Soil Columns for Measuring Solute Transport, Plant Growth and Soil Biota

Bio Protoc. 2021 Jan 20;11(2):e3884. doi: 10.21769/BioProtoc.3884.

Abstract

Researchers face a number of challenges in the construction of soil columns which can affect the outcome of their experiments. The use of intact soil cores closely mimics actual field conditions. However, the excavation of large intact soil cores is a time-consuming, labor-intensive process and may lead to soil compaction that would influence the solute transport behavior of the soil column. Repacked soil columns are used as an option to circumvent these challenges of intact soil cores. However, repacked soil columns also have their limitations and introduce other challenges. Here, we present a step by step procedure for the design of repacked soil columns to achieve a realistic bulk density, prevent preferential flow paths, and ensure hydraulic connectivity between soil layers. This protocol will be beneficial to Soil Scientists, Hydrologists and other Environmental Scientists utilizing repacked soil columns.

Keywords: Microbes; Nutrients; Soil columns; Solute transport; Unsaturated.