Development of phage-based assay to differentiate ciprofloxacin resistant and sensitive Salmonella Typhimurium

Nana Nguefang Laure; Juhee Ahn

doi:10.1007/s10068-020-00858-9

Development of phage-based assay to differentiate ciprofloxacin resistant and sensitive Salmonella Typhimurium

Food Sci Biotechnol. 2021 Jan 6;30(2):315-320. doi: 10.1007/s10068-020-00858-9. eCollection 2021 Feb.

Authors

Nana Nguefang Laure¹, Juhee Ahn¹

Affiliation

¹ Department of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Gangwon 24341 Republic of Korea.

Abstract

This study was designed to evaluate the possibility of using phage-amplification assay for discriminating between antibiotic-sensitive and antibiotic-resistant Salmonella Typhimurium. The characteristics of Salmonella phage PBST32 were determined by adsorption rate, one-step growth curve, and lytic activity. The ability of phage-based method to detect S. Typhimurium ATCC 19585 (ST^CIP) was determined in single culture and bacterial mixtures of S. Typhimurium ATCC 19585 (ST^WT), Klebsiella pneumoniae, and Staphylococcus aureus. The adsorption rates of PBST32 were 95% and 93% against ST^WT and ST^CIP after 20 min, respectively. The PBST32 showed latent period of 20 min and average burst size of 90 against ST^WT and ST^CIP. The ST^CIP was selectively detected in mixtures of S. aureus, K. pneumoniae, and ST^WT by phage amplification assay. These results provide useful information for designing phage amplification method that can differentially detect antibiotic-resistant pathogens.

Keywords: Antibiotic resistance; Ciprofloxacin; Detection; Foodborne pathogen; Phage.