MiR-129-5p Suppresses Cell Proliferation of Human Osteosarcoma Cancer by Down-Regulating LncRNA Lnc712

Yifan Yu; Wenshan Zuo; Wei Cai; Yong Xu; Weidong Liu; Zexue Zhao

doi:10.2147/CMAR.S284078

MiR-129-5p Suppresses Cell Proliferation of Human Osteosarcoma Cancer by Down-Regulating LncRNA Lnc712

Cancer Manag Res. 2021 Mar 10:13:2259-2264. doi: 10.2147/CMAR.S284078. eCollection 2021.

Authors

Yifan Yu¹, Wenshan Zuo¹, Wei Cai¹, Yong Xu¹, Weidong Liu¹, Zexue Zhao¹

Affiliation

¹ Department of Orthopaedics, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huai'an City, Nanjing Province, 223300, People's Republic of China.

Abstract

Introduction: Lnc712 has been characterized as an oncogenic lncRNA in breast cancer. This study aimed to investigate the role of Lnc712 in osteosarcoma (OS).

Methods: OS and paired non-tumor tissues were collected from 58 OS patients. Expression of Lnc712 and miR-129-5p in paired tissue samples was determined by RT-qPCR. Lnc712 and miR-129-5p expression was achieved in OS cells to study the interaction between them. Cell proliferation was analyzed by CCK-8 assay.

Results: Lnc712 was upregulated in OS and was inversely correlated with miR-129-5p. In OS cells, Lnc712 overexpression failed to significantly affect miR-129-5p, while miR-129-5p overexpression led to downregulated Lnc712. Cell proliferation showed that Lnc712 overexpression resulted in increased cell proliferation rate. MiR-129-5p overexpression played an opposite role and reversed the effect of Lnc712 overexpression.

Discussion: MiR-129-5p may suppress cell proliferation of OS by down-regulating Lnc712.

Keywords: Lnc712; miR-129-5p; osteosarcoma; proliferation.