The Histo-CLEM Workflow for tissues of model organisms

Abstract

Bridging from the macrostructure to the nanostructure of tissues is often technically challenging. To try to solve this, we developed a flexible CLEM workflow that can be applied to the analysis of tissues from diverse model organisms across various length scales. The Histo-CLEM Workflow combines three main microscopy techniques, namely histology, light microscopy and electron microscopy. Herein, all the steps of the Histo-CLEM Workflow are explained in detail to enable the adaptation of the method to tissue particularities and biological questions. The preparation and visualization of mice nerve fibers is shown as an application example of the presented Histo-CLEM Workflow.

Keywords: Correlative light and electron microscopy; Histology; Model organisms; Multimodal imaging; Tissues.