In situ printing of scaffolds for reconstruction of bone defects

Azadeh Mostafavi; Turdimuhammad Abdullah; Carina S Russell; Ebrahim Mostafavi; Tyrell J Williams; Numan Salah; Ahmed Alshahrie; Seth Harris; Seyed Masoud Moosavi Basri; Yogendra Kumar Mishra; Thomas J Webster; Adnan Memic; Ali Tamayol

doi:10.1016/j.actbio.2021.03.009

In situ printing of scaffolds for reconstruction of bone defects

Acta Biomater. 2021 Jun:127:313-326. doi: 10.1016/j.actbio.2021.03.009. Epub 2021 Mar 8.

Affiliations

¹ Department of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska, United States.
² Center of Nanotechnology, King Abdulaziz University, Jeddah, Saudi Arabia.
³ Department of Chemical Engineering, Northeastern University, Boston, Massachusetts, United States.
⁴ Veterinary Diagnostic Center, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, United States.
⁵ Department of Biomedical Engineering, American University of Sharjah, Sharjah, United Arab Emirates.
⁶ Mads Clausen Institute, NanoSYD, University of Southern Denmark, Sønderborg, Denmark.
⁷ Center of Nanotechnology, King Abdulaziz University, Jeddah, Saudi Arabia. Electronic address: amemic@kau.edu.sa.
⁸ Department of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska, United States; Department of Biomedical Engineering, University of Connecticut, Farmington, Connecticut, United States. Electronic address: atamayol@uchc.edu.

PMID: 33705990
DOI: 10.1016/j.actbio.2021.03.009

Abstract

Bone defects are commonly caused by traumatic injuries and tumor removal and critically sized defects overwhelm the regenerative capacity of the native tissue. Reparative strategies such as auto, xeno, and allografts have proven to be insufficient to reconstruct and regenerate these defects. For the first time, we introduce the use of handheld melt spun three dimensional printers that can deposit materials directly within the defect site to properly fill the cavity and form free-standing scaffolds. Engineered composite filaments were generated from poly(caprolactone) (PCL) doped with zinc oxide nanoparticles and hydroxyapatite microparticles. The use of PCL-based materials allowed low-temperature printing to avoid overheating of the surrounding tissues. The in situ printed scaffolds showed moderate adhesion to wet bone tissue, which can prevent scaffold dislocation. The printed scaffolds showed to be osteoconductive and supported the osteodifferentiation of mesenchymal stem cells. Biocompatibility of the scaffolds upon in vivo printing subcutaneously in mice showed promising results. STATEMENT OF SIGNIFICANCE.

Keywords: 3D printing; Bone defects; In vivo printing; Melt spinning; Osteogenic.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Regeneration
Bone and Bones
Durapatite
Mice
Osteogenesis
Polyesters
Printing, Three-Dimensional*
Tissue Engineering
Tissue Scaffolds*

Substances

Polyesters
Durapatite

Grants and funding

R01 AR073822/AR/NIAMS NIH HHS/United States