Objective: To investigate the inhibitory effect and molecular mechanism of microRNA-30d (miR-30d) in the process of proliferation, migration and invasion of malignant mesothelioma cell line MSTO-211H. Methods: In April 2017, the human MSTO-211H cells was used to establish miR-30d overexpressed MSTO-211H cell model by transfection of miR-30d mimics. The qRT-PCR was performed to detect the expression level of miR-30d in the cells transfected miR-30d mimics. The effects of miR-30d on the proliferation, apoptosis, migration and invasion of MSTO-211H cells were analyzed by CCK-8 experiment, flow cytometry, cell scratch experiment and Transwell method. Results: After transfection of miR-30d, the expression level of miR-30d in the MSTO-211H+miR-30d cells group was significantly higher than MSTO-211H+miR NC cells group (P<0.01) . The cell activity of MSTO-211H+miR-30d group (105.13%±2.35%) was significantly lower than MSTO-211H+miR NC cells group (115.40%±1.35%) , and the level of apoptosis (3.97%±0.36%) was significantly higher than MSTO-211H+miR NC cells group (1.47%±0.10%) (P<0.01) . The relative migration areas at 12 and 24 h of MSTO-211H+miR-30d cells group (9.35±3.16 μm(2) and 58.19±1.82 μm(2)) were significantly lower than MSTO-211H+miR NC cells group (54.42±5.26 μm(2) and 88.32±1.96 μm(2)) (P<0.01) . Compared with the MSTO-211H+miR NC cells group, the numbers of cell migration and cell invasion were reduced in the MSTO-211H+miR-30d cells group (P<0.01) . Conclusion: miR-30d can regulate the progression of malignant pleural mesothelioma by inhibiting the proliferation, apoptosis, migration and invasion of MSTO-211H cells.
目的： 探究microRNA-30d（miR-30d）对人恶性胸膜间皮瘤细胞MSTO-211H的增殖、迁移和侵袭的抑制作用。 方法： 于2017年4月，以MSTO-211H细胞为模型，通过转染miR-30d模拟物（miR-30d mimics）建立miR-30d过表达的MSTO-211H细胞模型，利用qRT-PCR检测转染后细胞miR-30d的表达水平，通过CCK-8实验、流式细胞术、细胞划痕实验以及Transwell法分析miR-30d对MSTO-211H细胞增殖、凋亡、迁移和侵袭等表型的影响。 结果： 转染miR-30d mimics后，MSTO-211H+miR-30d组细胞中miR-30d表达水平明显高于MSTO-211H+miR NC组细胞（P<0.01）。MSTO-211H+miR-30d组细胞活性（105.13%±2.35%）显著低于MSTO-211H+miR NC组（115.40%±1.35%），凋亡水平（3.97%±0.36%）显著高于MSTO-211H+miR NC组（1.47%±0.10%）（P<0.01）。MSTO-211H+miR-30d组细胞在12、24 h的相对迁移面积分别为9.35±3.16 μm(2)和58.19±1.82 μm(2)，显著低于MSTO-211H+miR NC组（54.42±5.26 μm(2)和88.32±1.96 μm(2)）（P<0.01）。与MSTO-211H+miR NC组比较，MSTO-211H+miR-30d组细胞的迁移和侵袭细胞数显著减少（P<0.01）。 结论： miR-30d能通过抑制MSTO-211H细胞的增殖、凋亡、迁移和侵袭进而调控恶性胸膜间皮瘤的进展。.
Keywords: Apoptosis; Cell migration; Cell proliferation; Invasion; Malignant pleural mesothelioma; miR-30d.