A Q-switched, high-energy pulsed master oscillator power amplifier fiber laser utilizing the lab-built 100/400 µm double-cladding Yb-doped fiber is demonstrated. After two-stage amplification, the pulse energy was boosted to 25.5 mJ, for an average power of 510 W at a repetition of 20 kHz, yielding a slope efficiency of approximately 72.8%; the pulse duration was approximately 140 ns, and corresponding peak power was 182.1 kW. What is more, the limitation of further promotion of pulse energy was proposed: the threshold-like parasitic oscillation, which was determined by the injecting power, repetition, and fiber length, was the main restriction on power scaling in ultra-high-energy systems. Efficient solutions were proposed to suppress the parasitic oscillation by experimentally studies.