In vivo treatment with a subacute low dose of 3-nitropropionic acid does not induce genotoxicity or mutagenicity in rats

Flavia Tasmin Techera Antunes; Emanuelle Sistherenn Caminski; Jaqueline Nascimento Picada; Andrea Pereira Regner; Eliane Dallegrave; Alessandra Hubner de Souza

doi:10.1016/j.toxicon.2021.03.001

In vivo treatment with a subacute low dose of 3-nitropropionic acid does not induce genotoxicity or mutagenicity in rats

Toxicon. 2021 May:195:20-23. doi: 10.1016/j.toxicon.2021.03.001. Epub 2021 Mar 6.

Authors

Flavia Tasmin Techera Antunes¹, Emanuelle Sistherenn Caminski², Jaqueline Nascimento Picada³, Andrea Pereira Regner⁴, Eliane Dallegrave⁵, Alessandra Hubner de Souza⁶

Affiliations

¹ Program of Postgraduation in Cellular and Molecular Biology Applied to Health, Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil. Electronic address: tas.min@rede.ulbra.br.
² Department of Pharmacoscience, Federal University Science of Health of Porto Alegre (UFCSPA), Rio Grande do Sul, Brazil. Electronic address: emanuelle@ufcspa.edu.br.
³ Laboratory of Genetic Toxicology, Lutheran University of Brazil, Canoas, RS, Brazil. Electronic address: Jaqueline.picada@ulbra.br.
⁴ Program of Postgraduation in Cellular and Molecular Biology Applied to Health, Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil. Electronic address: andrea.regner@ulbra.br.
⁵ Department of Pharmacoscience, Federal University Science of Health of Porto Alegre (UFCSPA), Rio Grande do Sul, Brazil. Electronic address: elianedal@ufcspa.edu.br.
⁶ Program of Postgraduation in Cellular and Molecular Biology Applied to Health, Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil. Electronic address: alessandrahubnersouza@gmail.com.

PMID: 33689791
DOI: 10.1016/j.toxicon.2021.03.001

Abstract

3-nitropropionic acid (3-NP) is a toxin that causes neural damage in the striatum and can lead to the development of Huntington's disease manifestations in animal models. Several studies have shown genotoxicity related to the 3-NP treatment. This study investigated potential genotoxicity and mutagenicity that was induced by a low dose (6.25 mg/kg i. p.) 3-NP subacute treatment (daily, over 6 days) in a rat model. The arterial blood and the frontal cortex were analyzed by the comet assay and the bone marrow by micronucleus. Surprisingly, the 3-NP subacute treatment with the low dose did not show genotoxic or mutagenic effects.

Keywords: Comet assay; Huntington's disease; Micronucleus; Polychromatic erythrocytes.

MeSH terms

Animals
Comet Assay
DNA Damage*
Dose-Response Relationship, Drug
Micronucleus Tests
Mutagenicity Tests
Mutagens* / toxicity
Nitro Compounds / toxicity*
Propionates / toxicity*
Rats

Substances

Mutagens
Nitro Compounds
Propionates
3-nitropropionic acid