3D Organoid Culture Using Skin Keratinocytes Derived from Human Induced Pluripotent Stem Cells

Tomoko Miyake; Mikio Shimada

doi:10.1007/7651_2021_357

3D Organoid Culture Using Skin Keratinocytes Derived from Human Induced Pluripotent Stem Cells

Methods Mol Biol. 2022:2454:285-295. doi: 10.1007/7651_2021_357.

Authors

Tomoko Miyake¹, Mikio Shimada²

Affiliations

¹ Cosmetic R&D Department, Takara Belmont Corp., Tokyo, Japan.
² Laboratory for Advanced Nuclear Energy, Institute of Innovative Research, Tokyo Institute of Technology, Tokyo, Japan. mshimada@lane.iir.titech.ac.jp.

PMID: 33689165
DOI: 10.1007/7651_2021_357

Abstract

The keratinocytes are predominant cells in the epidermis of the human skin. To assess the cellular response of the keratinocytes to the genotoxic stress, we derived the skin keratinocytes from human induced pluripotent stem cells (iPSCs). Furthermore, three-dimensional (3D) organoid culture method is powerful tool to analyze the organ and tissue response against the genotoxic stress. Here we describe the method of 3D organoid culture using skin keratinocytes derived from human iPSCs.

Keywords: 3D organoid culture; Epidermis; Genotoxic stress; Human iPS cells; Skin keratinocytes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation / physiology
Fibroblasts
Humans
Induced Pluripotent Stem Cells*
Keratinocytes
Organoids
Skin