PAMK Relieves LPS-Induced Enteritis and Improves Intestinal Flora Disorder in Goslings

Wanyan Li; Xuelian Xiang; Bingxin Li; Yifei Wang; Long Qian; Yunbo Tian; Yunmao Huang; Danning Xu; Nan Cao

doi:10.1155/2021/9721353

PAMK Relieves LPS-Induced Enteritis and Improves Intestinal Flora Disorder in Goslings

Evid Based Complement Alternat Med. 2021 Feb 22:2021:9721353. doi: 10.1155/2021/9721353. eCollection 2021.

Authors

Wanyan Li^{1

2}, Xuelian Xiang^{1

2}, Bingxin Li^{1

2}, Yifei Wang^{1

2}, Long Qian^{1

2}, Yunbo Tian^{1

2}, Yunmao Huang^{1

2}, Danning Xu^{1

2}, Nan Cao^{1

2}

Affiliations

¹ College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
² Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou 510225, China.

Abstract

Polysaccharide of Atractylodes macrocephala Koidz (PAMK) is a biologically active component of Atractylodes macrocephala, which has the effect of maintaining the immune homeostasis of the body. Therefore, this study constructed a model of PAMK to relieve LPS-induced gosling enteritis and observed the morphological changes of the small intestine after HE staining. ELISA was used to detect serum CRP, IL-1β, IL-6, and TNF-α levels; immunohistochemistry was used to detect the positive rate of IgA in the small intestine; TLR4, occludin, ZO-1, cytokines, and immunoglobulin mRNA expression in the small intestine were detected by qPCR; and intestinal flora of gosling excrement was analyzed by 16S rDNA sequencing to analyze the protective effect of PAMK on goslings enteritis and the impact on intestinal flora. The results showed that PAMK relieves LPS-induced gosling enteritis by maintaining the small intestine morphology, cytokine, tight junctions, and immunoglobulin relatively stable and improving the disorder of intestinal flora.