A highly sensitive and selective fluorescence turn-on probe for the sensing of H2S in vitro and in vivo

Qi Sun; Heng Liu; Yuan Qiu; Jun Chen; Feng-Shou Wu; Xiao-Gang Luo; Da-Wei Wang

doi:10.1016/j.saa.2021.119620

A highly sensitive and selective fluorescence turn-on probe for the sensing of H₂S in vitro and in vivo

Spectrochim Acta A Mol Biomol Spectrosc. 2021 Jun 5:254:119620. doi: 10.1016/j.saa.2021.119620. Epub 2021 Feb 26.

Authors

Qi Sun¹, Heng Liu², Yuan Qiu¹, Jun Chen¹, Feng-Shou Wu¹, Xiao-Gang Luo³, Da-Wei Wang⁴

Affiliations

¹ Key Laboratory for Green Chemical Process of Ministry of Education and School of Chemistry and School of Chemistry and Environmental Engineering, Wuhan Institute of Technology, Wuhan 430205, China.
² Institute of Functional Materials and Molecular Imaging, Key Laboratory of Emergency and Trauma, Ministry of Education, Key Laboratory of Hainan Trauma and Disaster Rescue, College of Clinical Medicine, College of Emergency and Trauma, Hainan Medical University, Haikou 571199, China.
³ Key Laboratory for Green Chemical Process of Ministry of Education and School of Chemistry and School of Chemistry and Environmental Engineering, Wuhan Institute of Technology, Wuhan 430205, China; School of Materials Science and Engineering, Zhengzhou University, No.100 Science Avenue, Zhengzhou City 450001, Henan Province, China.
⁴ State Key Laboratory of Elemento-Organic Chemistry, and Department of Chemical Biology, National Pesticide Engineering Research Center, Collaborative Innovation Center of Chemical Science and Engineering, College of Chemistry, Nankai University, Tianjin 300071, China. Electronic address: wangdw@nankai.edu.cn.

PMID: 33684854
DOI: 10.1016/j.saa.2021.119620

Abstract

A fluorescence turn-on probe, 2-butyl-1,3-dioxo-2,3-dihydro-1H-benzo[de]isoquinolin-6-yl 2,4-dinitrobenzenesulfonate (NT-SH), has been constructed for sensing of hydrogen sulfide (H₂S). NT-SH exhibited excellent detection performance including favorable water solubility, low fluorescence background, high enhancement (45-fold), large linear response range (0-50 μM) and low detection limit (80.01 nM) for H₂S in aqueous. In addition, the response mechanism of NT-SH for H₂S was confirmed by the theoretical calculation and mass spectral analysis. More importantly, the imaging experiments of H₂S in vitro and in vivo confirmed that NT-SH had low cytotoxicity, and favorable biocompatibility. In addition, it illustrated that NT-SH was able to detected exogenous H₂S in living cells and zebrafish. These results suggested that NT-SH can be act as a potential molecular tool for detecting of H₂S in aqueous solution, in vitro and in vivo.

Keywords: Bioimaging; Fluorescent probe; Hydrogen sulfide; Zebrafish.

MeSH terms

Animals
Fluorescent Dyes*
HeLa Cells
Humans
Hydrogen Sulfide*
Spectrometry, Fluorescence
Zebrafish

Substances

Fluorescent Dyes
Hydrogen Sulfide