Evaluation of biological and chemical additives on microbial community, fermentation characteristics, aerobic stability, and in vitro gas production of SuMu No. 2 elephant grass

J Sci Food Agric. 2021 Oct;101(13):5429-5436. doi: 10.1002/jsfa.11191. Epub 2021 Mar 19.

Abstract

Background: The study was conducted to evaluate the effects of biological and chemical additives on microbial community, fermentation characteristics, aerobic stability, and in vitro gas production of SuMu No. 2 elephant grass.

Results: Aerobic bacteria and yeast were not affected on days 5 and 7 but were significantly (P < 0.224) reduced on days 14, 30, and 60, whereas lactic acid and lactic acid bacteria were significantly (P > 0.001) higher in all ensiling days within all treatment groups. During the ensiling days, the pH, acetic acid, butyric acid, and yeast were decreased in all treatment groups, whereas the Lactobacillus plantarum group and L. plantarum + natamycin group were highly significantly (P > 0.001) decreased. During air exposure, the water-soluble carbohydrates, ammonia nitrogen, lactic acid, and acetic acid were not affected on days 1-4, whereas pH and aerobic bacteria (were significantly (P < 0.05) increased on days 2-4. The addition of Lactobacillus plantarum and natamycin increased the gas production, in vitro dry matter digestibility, and in vitro neutral detergent fiber of SuMu No. 2 elephant grass silages.

Conclusions: The addition of biological and chemical additives, such as L. plantrum alone and the combination with natamycin, affected the undesirable microbial community, fermentation characteristics, aerobic stability, and in vitro gas of SuMu No. 2 elephant grass. © 2021 Society of Chemical Industry.

Keywords: SuMu No. 2 elephant grass; aerobic stability; fermentation quality; in vitro gas production; microbiota analysis.

MeSH terms

  • Acetic Acid / analysis
  • Acetic Acid / metabolism
  • Aerobiosis
  • Bacteria / classification
  • Bacteria / genetics
  • Bacteria / isolation & purification
  • Bacteria / metabolism*
  • Fermentation
  • Gases / analysis
  • Gases / metabolism*
  • Lactic Acid / analysis
  • Lactic Acid / metabolism
  • Lactobacillus plantarum / metabolism
  • Microbiota*
  • Natamycin / analysis
  • Natamycin / metabolism
  • Pennisetum / chemistry
  • Pennisetum / microbiology*
  • Silage / analysis
  • Silage / microbiology

Substances

  • Gases
  • Lactic Acid
  • Natamycin
  • Acetic Acid