Fluorescent bacterial biosensor E. coli/pTdcR-TurboYFP sensitive to terahertz radiation

Danil S Serdyukov; Tatiana N Goryachkovskaya; Irina A Mescheryakova; Sergei A Kuznetsov; Vasiliy M Popik; Sergey E Peltek

doi:10.1364/BOE.412074

Fluorescent bacterial biosensor E. coli/pTdcR-TurboYFP sensitive to terahertz radiation

Biomed Opt Express. 2021 Jan 5;12(2):705-721. doi: 10.1364/BOE.412074. eCollection 2021 Feb 1.

Authors

Danil S Serdyukov^{1

2

3}, Tatiana N Goryachkovskaya^{1

2}, Irina A Mescheryakova^{1

2}, Sergei A Kuznetsov^{4

5}, Vasiliy M Popik⁶, Sergey E Peltek^{1

2}

Affiliations

¹ Laboratory of Molecular Biotechnologies of Federal research center Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, 10 Lavrentiev Aven., Novosibirsk, 630090, Russia.
² Kurchatov Genomics Center of Federal research center Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, 10 Lavrentiev Aven., Novosibirsk, 630090, Russia.
³ Institute of Laser Physics of the Siberian Branch of the Russian Academy of Sciences, 15B Lavrentiev Aven., Novosibirsk, 630090, Russia.
⁴ Physics Department of Novosibirsk State University, 2 Pirogov Str., Novosibirsk, 630090, Russia.
⁵ Technological Design Institute of Applied Microelectronics - Novosibirsk Branch of Rzhanov Institute of Semiconductor Physics of the Siberian Branch of the Russian Academy of Sciences, 2/1 Lavrentiev Aven., Novosibirsk, 630090, Russia.
⁶ Budker Institute of Nuclear Physics of the Siberian Branch of the Russian Academy of Sciences, 11 Lavrentiev Aven., Novosibirsk, 630090, Russia.

Abstract

A fluorescent biosensor E. coli/pTdcR-TurboYFP sensitive to terahertz (THz) radiation was developed via transformation of Escherichia coli (E. coli) cells with plasmid, in which the promotor of the tdcR gene controls the expression of yellow fluorescent protein TurboYFP. The biosensor was exposed to THz radiation in various vessels and nutrient media. The threshold and dynamics of fluorescence were found to depend on irradiation conditions. Heat shock or chemical stress yielded the absence of fluorescence induction. The biosensor is applicable to studying influence of THz radiation on the activity of tdcR promotor that is involved in the transport and metabolism of threonine and serine in E. coli.