Integrated Bioinformatics Analysis Exhibits Pivotal Exercise-Induced Genes and Corresponding Pathways in Malignant Melanoma

Jun Zhu; Suyu Hao; Xinyue Zhang; Jingyue Qiu; Qin Xuan; Liping Ye

doi:10.3389/fgene.2020.637320

Integrated Bioinformatics Analysis Exhibits Pivotal Exercise-Induced Genes and Corresponding Pathways in Malignant Melanoma

Front Genet. 2021 Feb 18:11:637320. doi: 10.3389/fgene.2020.637320. eCollection 2020.

Authors

Jun Zhu¹, Suyu Hao², Xinyue Zhang³, Jingyue Qiu⁴, Qin Xuan⁵, Liping Ye⁶

Affiliations

¹ Administrative Office, Shanghai Basilica Clinic, Shanghai, China.
² Shuangwu Information Technical Company Ltd., Shanghai, China.
³ School of Education, Hangzhou Normal University, Hangzhou, China.
⁴ School of Physical Science and Engineering, East China University of Science and Technology, Shanghai, China.
⁵ School of Sports Science and Engineering, East China University of Science and Technology, Shanghai, China.
⁶ Department of Clinical Nursing, Minhang Hospital, Fudan University, Shanghai, China.

Abstract

Malignant melanoma represents a sort of neoplasm deriving from melanocytes or cells developing from melanocytes. The balance of energy and energy-associated body composition and body mass index could be altered by exercise, thereby directly affecting the microenvironment of neoplasm. However, few studies have examined the mechanism of genes induced by exercise and the pathways involved in melanoma. This study used three separate datasets to perform comprehensive bioinformatics analysis and then screened the probable genes and pathways in the process of exercise-promoted melanoma. In total, 1,627 differentially expressed genes (DEGs) induced by exercise were recognized. All selected genes were largely enriched in NF-kappa B, Chemokine signaling pathways, and the immune response after gene set enrichment analysis. The protein-protein interaction network was applied to excavate DEGs and identified the most relevant and pivotal genes. The top 6 hub genes (Itgb2, Wdfy4, Itgam, Cybb, Mmp2, and Parp14) were identified, and importantly, 5 hub genes (Itgb2, Wdfy4, Itgam, Cybb, and Parp14) were related to weak disease-free survival and overall survival (OS). In conclusion, our findings demonstrate the prognostic value of exercise-induced genes and uncovered the pathways of these genes in melanoma, implying that these genes might act as prognostic biomarkers for melanoma.

Keywords: Disease-free survival; exercise; integrated bioinformatics analysis; malignant melanoma; prognosis.