Insight into Cr(VI) biosorption onto algal-bacterial granular sludge: Cr(VI) bioreduction and its intracellular accumulation in addition to the effects of environmental factors

Abstract

Hexavalent chromium (Cr(VI)) is one of the typical heavy metals that pose a great threat to the environment. As a novel biotechnology, algal-bacterial aerobic granular sludge (AGS) possesses the merits of both bacterial AGS and algae. This study firstly evaluated Cr(VI) removal via biosorption by algal-bacterial AGS under different operation conditions and then some environmental factors. Results show that the highest Cr(VI) reduction (99.3%) and total Cr removal (89.1%) were achieved within 6 h at pH 2 and 6, respectively. The coexisting oxyanions exhibited slight effects, while both tested natural organic matters (humic acid and tannic acid) and carbon sources promoted Cr(VI) reduction at some appropriate concentrations. The coexistence of metal cations favored Cr(VI) reduction, achieving the highest enhancement of 8.1% by Cu²⁺ at 5 mg/L, while the total Cr removal was suppressed to some extent. Salinity > 5 g/L severely inhibited both Cr(VI) reduction and total Cr removal. Moreover, the loaded Cr in algal-bacterial AGS was found to be almost in the form of Cr(III), with 66.8% being contributed by intracellular accumulation. This work suggests that Cr(VI) reduction and intracellular accumulation are the main mechanisms involved in Cr(IV) biosorption onto algal-bacterial AGS.

Keywords: Algal-bacterial aerobic granular sludge; Bioreduction; Cr(VI) removal; Environmental factors; Intracellular accumulation.