Transfer of sulfidized silver from silver nanoparticles, in sewage sludge, to plants and primary consumers in agricultural soil environment

Abstract

Consumer products containing silver nanoparticles (AgNPs) release silver (Ag) to the environment, particularly wastewater. Sewage sludge (SS), which contains numerous contaminants including Ag, is recycled by spreading on agricultural land. Although slight impacts and bioaccumulation of Ag sulfide (Ag₂S, the main species found in SS) in terrestrial organisms have been demonstrated, possible trophic transfer into plants and subsequently animal species has not been examined. Accordingly, the present study experimentally measured the transfer of Ag from AgNPs and sulfidized Ag into plants and primary consumers and compared their bioavailability. Nine plant cultivars were grown in soil mixed with SS containing Ag, which revealed that bioaccumulation of Ag by plants is species-dependent. Ryegrass (the plant species with the greatest accumulation - up to 0.2 mg kg^-1) was then cultivated on a larger scale to expose snails and locusts for several weeks. While locusts did not accumulate Ag after two weeks of exposure, snails exhibited Ag bioaccumulation after 5 weeks when soil was accessible. Sulfidized Ag derived from AgNPs were less available (bioaccumulation up to 2.5 mg kg^-1) than the Ag from the original AgNPs (bioaccumulation up to 15 mg kg^-1). This transfer potential of Ag could have consequences for food webs due to chronic exposure linked to SS spreading practices. This study shows that transformations of AgNPs in treatment plants attenuate but do not completely eliminate the risk of Ag to plant and animal species SS.

Keywords: Bioaccumulation; Food chain; Locusts; Ryegrass; Snails; Sulfidized silver.