Influences of dietary vitamin D3 on growth, antioxidant capacity, immunity and molting of Chinese mitten crab (Eriocheir sinensis) larvae

Abstract

This study investigates the effects of vitamin D₃ (VD₃) on growth performance, antioxidant capacity, immunity and molting of larval Chinese mitten crab Eriocheir sinensis. A total of 6,000 larvae (7.52 ± 0.10 mg) were fed with six isonitrogenous and isolipidic experimental diets with different levels of dietary VD₃ (0, 3000, 6000, 9000, 12000 and 36000 IU/kg) respectively for 23 days. The highest survival and molting frequency were found in crabs fed 6000 IU/kg VD₃. Weight gain, specific growth rate, and carapace growth significantly increased in crabs fed 3000 and 6000 IU/kg VD₃ compared to the control. Broken-line analysis of molting frequency, weight gain and specific growth rate against dietary VD₃ levels indicates that the optimal VD₃ requirement for larval crabs is 4825-5918 IU/kg. The highest whole-body VD₃ content occurred in the 12000 IU/kg VD₃ group, and the 25-dihydroxy VD₃ content decreased with the increase of dietary VD₃. The malonaldehyde content was lower than the control. Moreover, the superoxide dismutase activity, glutathione peroxidase and total antioxidant capacity of crab fed 6000 IU/kg VD₃ were significantly higher than in control. Crabs fed 9000 IU/kg showed the highest survival after 120 h of salinity stress, and the relative mRNA expressions indicate vitamin D receptor (VDR) is the important regulatory element in molting and innate immunity. The molting-related gene expressions showed that the response of crab to salinity was self-protective. This study would contribute to a new understanding of the molecular basis underlying molting and innate immunity regulation by vitamin D₃ in E. sinensis.

Keywords: Eriocheir sinensis; Immunity; Molting; Salinity stress; Vitamin D(3).