Establishment of in vitro 3D spheroid cell cultivation from human gynecologic cancer tissues

Haruka Ueda; Yutaro Mori; Kaoru Yamawaki; Tatsuya Ishiguro; Hirokazu Ohata; Ai Sato; Kentaro Sugino; Nozomi Yachida; Manako Yamaguchi; Kazuaki Suda; Ryo Tamura; Kosuke Yoshihara; Koji Okamoto; Takayuki Enomoto

doi:10.1016/j.xpro.2021.100354

Establishment of in vitro 3D spheroid cell cultivation from human gynecologic cancer tissues

STAR Protoc. 2021 Feb 18;2(1):100354. doi: 10.1016/j.xpro.2021.100354. eCollection 2021 Mar 19.

Affiliations

¹ Department of Obstetrics and Gynecology, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-dori, Niigata, Niigata 951-8510, Japan.
² Division of Cancer Differentiation, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan.

Abstract

Advanced-stage gynecologic cancer remains a life-threatening disease. Here, we present a protocol for establishment of stable in vitro 3D spheroid cells derived from human uterine endometrial and ovarian cancer tissues. The tumor-derived spheroid cells have cancer stem cell-related characteristics, including tumorigenesis, and can be used for biological and biochemical analyses and drug efficacy assays. Because these cells possess the biological characteristics of original human tumors, spheroid cells and spheroid-derived xenografts will have applications in personalized medicine in the future. For complete details on the use and execution of this protocol, please refer to Ishiguro et al. (2016) and Mori et al. (2019).

Keywords: Cancer; Cell culture; Stem cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques*
Female
Humans
Ovarian Neoplasms* / metabolism
Ovarian Neoplasms* / pathology
Spheroids, Cellular* / metabolism
Spheroids, Cellular* / pathology
Tumor Cells, Cultured
Uterine Neoplasms* / metabolism
Uterine Neoplasms* / pathology