The chloroplast genome of a unicellular green alga strain isolated from the rubber processing wastewater

Bingying Han; Yaojia Mu; Deguan Tan; Shuai Ma; Lili Fu; Xuepiao Sun; Jiaming Zhang

doi:10.1080/23802359.2020.1844090

The chloroplast genome of a unicellular green alga strain isolated from the rubber processing wastewater

Mitochondrial DNA B Resour. 2021 Jan 21;6(1):15-16. doi: 10.1080/23802359.2020.1844090.

Authors

Bingying Han¹, Yaojia Mu², Deguan Tan¹, Shuai Ma¹, Lili Fu¹, Xuepiao Sun¹, Jiaming Zhang^{1

2

3}

Affiliations

¹ Institute of Tropical Bioscience and Biotechnology, MOA Key Laboratory of Tropical Crops Biology and Genetic Resources, Hainan Bioenergy Center, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
² College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
³ Hainan Institute for Tropical Agricultural Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.

Abstract

Chlorella vulgaris ITBBA3-12 has a role in the purification of the rubber processing wastewater. Its complete chloroplast genome contains 168369 bp, with a G + C content of 33.0%. A total of 147 genes were annotated, including 113 protein-coding genes, three rRNA (rrn23, rrn16, and rrn5) genes, and 31 tRNA genes. The significant feature of the chloroplast genome is that the genes encoding subunit V (petG), VI (petL), and apocytochrome f (petA) of the cytochrome b6/f complex are in triplicate, which was not observed in the other C. vulgaris strains. Phylogenetic analysis using the chloroplast genomes of Chlorophyta species indicated that ITBBA3-12 is closely related to C. vulgaris strain UTEX259 and NJ-7, and they clustered in the Chlorella lineage.

Keywords: Chlorella; complete chloroplast genome; rubber; wastewater.

Grants and funding

This research was supported in part by the Natural Science Foundation of Hainan Province [318MS091 and 2019RC296], and the Natural Science Foundation of China [31700195 and 31800271].