In-vitro GLP-1 Release Assay Using STC-1 Cells

Liu Qi; Tan Shuai; Jia Da; Burstein Ezra; Sifuentes-Dominguez Luis

doi:10.21769/BioProtoc.3717

In-vitro GLP-1 Release Assay Using STC-1 Cells

Bio Protoc. 2020 Aug 20;10(16):e3717. doi: 10.21769/BioProtoc.3717.

Authors

Liu Qi¹, Tan Shuai¹, Jia Da², Burstein Ezra¹, Sifuentes-Dominguez Luis³

Affiliations

¹ Department of Internal Medicine, Division of Digestive and Liver Diseases, University of Texas Southwestern Medical Center, Dallas, United States.
² Key Laboratory of Birth Defects and Related Diseases of Women and Children, Department of Pediatrics West China Second University Hospital, State Key Laboratory of Biotherapy and Collaborative Innovation Center of Biotherapy, Sichuan University, Chengdu, China.
³ Department of Pediatrics, Division of Pediatric Gastroenterology, Hepatology and Nutrition, University of Texas Southwestern Medical Center, Dallas, United States.

Abstract

Enteroendocrine cells (EECs) are known chemosensors in the gastrointestinal (GI) epithelium. They release a diversity of gut hormones in response to various stimuli. Here, we report an in-vitro assay to measure GLP-1 release from cultured murine EEC's under fatty acid stimulation.

Keywords: Docosahexaenoic acid (DHA); Enteroendocrine cells; Glucagon-like peptide-1 (GLP-1); L-cells; STC-1.