Direct measurement of the scattering coefficient

Biomed Opt Express. 2020 Dec 14;12(1):320-335. doi: 10.1364/BOE.410248. eCollection 2021 Jan 1.

Abstract

In general, the measurement of the main three optical properties (µ a , µ s and g) in turbid media requires a very precise measurement of the total transmission (TT), the total reflection (TR) and the collimated transmission (CT). Furthermore, an inverse algorithm such as inverse adding doubling or inverse Monte-Carlo-simulations is required for the reconstruction of the optical properties. Despite many available methods, the error free measurement of the scattering coefficient or the g-factor still remains challenging. In this study, we present a way to directly calculate the scattering coefficient from the total and collimated transmission. To allow this, it can be shown that T T C T is proportional to e μ s d for a wide range of optical properties if the sample is thick enough. Moreover, a set-up is developed and validated to measure the collimated transmission precisely.